Detailed information of TA system TA065960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5392679..5393265
• Replicon: chromosome
• Accession: NZ_CP017099
• Organism: Pseudomonas aeruginosa strain DN1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: BG483_RS25905
• Protein ID: WP_003120987.1
• Coordinates: 5392966..5393265 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: BG483_RS25900
• Protein ID: WP_003448662.1
• Coordinates: 5392679..5392969 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG483_RS25885	5388261..5390150	+	1890	WP_023101908.1	hypothetical protein	-
BG483_RS25890	5390147..5392123	+	1977	WP_031693914.1	DEAD/DEAH box helicase	-
BG483_RS25895	5392264..5392608	+	345	WP_016851612.1	hypothetical protein	-
BG483_RS25900	5392679..5392969	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
BG483_RS25905	5392966..5393265	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BG483_RS25910	5393467..5394591	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
BG483_RS25915	5394591..5396300	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
BG483_RS25920	5396304..5397629	+	1326	WP_058343417.1	type 4b pilus protein PilO2	-
BG483_RS25925	5397619..5398152	+	534	WP_023875934.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5362518..5455159	92641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T65960 WP_003120987.1 NZ_CP017099:c5393265-5392966 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T65960 NZ_CP017099:c5393265-5392966 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT65960 WP_003448662.1 NZ_CP017099:c5392969-5392679 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT65960 NZ_CP017099:c5392969-5392679 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73