65959

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5270576..5271190 Replicon chromosome
Accession NZ_CP017099
Organism Pseudomonas aeruginosa strain DN1

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag BG483_RS25325 Protein ID WP_071534354.1
Coordinates 5271008..5271190 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag BG483_RS25320 Protein ID WP_012077229.1
Coordinates 5270576..5270980 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG483_RS25295 5266716..5267426 + 711 WP_012074129.1 hypothetical protein -
BG483_RS25300 5267423..5268343 + 921 WP_012074128.1 hypothetical protein -
BG483_RS25305 5268340..5268879 + 540 WP_012074127.1 hypothetical protein -
BG483_RS25310 5268879..5269577 + 699 WP_033896049.1 hypothetical protein -
BG483_RS25315 5269562..5270533 + 972 WP_012077228.1 hypothetical protein -
BG483_RS25320 5270576..5270980 - 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG483_RS25325 5271008..5271190 - 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
BG483_RS25330 5271733..5272665 - 933 WP_003120918.1 ZIP family metal transporter -
BG483_RS25335 5272684..5273295 - 612 WP_003098862.1 superoxide dismutase -
BG483_RS25340 5273308..5273757 - 450 WP_003094369.1 hypothetical protein -
BG483_RS25345 5273785..5275161 - 1377 WP_023101553.1 class II fumarate hydratase -
BG483_RS25350 5275154..5275549 - 396 WP_003094374.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5237533..5271190 33657


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T65959 WP_071534354.1 NZ_CP017099:c5271190-5271008 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T65959 NZ_CP017099:c5271190-5271008 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT65959 WP_012077229.1 NZ_CP017099:c5270980-5270576 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT65959 NZ_CP017099:c5270980-5270576 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References