Detailed information of TA system TA065951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2739176..2739705
• Replicon: chromosome
• Accession: NZ_CP017094
• Organism: Staphylococcus aureus strain 2148.C01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A7U45_RS14265
• Protein ID: WP_000621175.1
• Coordinates: 2739343..2739705 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: A7U45_RS14260
• Protein ID: WP_000948331.1
• Coordinates: 2739176..2739346 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U45_RS14230	2734213..2734773	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
A7U45_RS14235	2734981..2735460	+	480	WP_001287088.1	hypothetical protein	-
A7U45_RS14240	2735453..2737036	+	1584	WP_001294626.1	PH domain-containing protein	-
A7U45_RS14245	2737023..2737514	+	492	WP_001205910.1	PH domain-containing protein	-
A7U45_RS14250	2737518..2737877	+	360	WP_000581200.1	holo-ACP synthase	-
A7U45_RS14255	2737943..2739091	+	1149	WP_001281145.1	alanine racemase	-
A7U45_RS14260	2739176..2739346	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A7U45_RS14265	2739343..2739705	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7U45_RS14275	2740054..2741055	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
A7U45_RS14280	2741174..2741500	+	327	WP_001052491.1	anti-sigma factor antagonist	-
A7U45_RS14285	2741502..2741981	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
A7U45_RS14290	2741956..2742726	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T65951 WP_000621175.1 NZ_CP017094:2739343-2739705 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T65951 NZ_CP017094:2739343-2739705 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT65951 WP_000948331.1 NZ_CP017094:2739176-2739346 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT65951 NZ_CP017094:2739176-2739346 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1