Detailed information of TA system TA065918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2165647..2166176
• Replicon: chromosome
• Accession: NZ_CP017090
• Organism: Staphylococcus aureus strain ISU935 isolate ST5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A2V17_RS11100
• Protein ID: WP_000621175.1
• Coordinates: 2165647..2166009 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: A2V17_RS11105
• Protein ID: WP_000948331.1
• Coordinates: 2166006..2166176 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2V17_RS11075	2162626..2163396	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
A2V17_RS11080	2163371..2163850	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
A2V17_RS11085	2163852..2164178	-	327	WP_001052491.1	anti-sigma factor antagonist	-
A2V17_RS11090	2164297..2165298	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
A2V17_RS11100	2165647..2166009	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A2V17_RS11105	2166006..2166176	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A2V17_RS11110	2166261..2167409	-	1149	WP_001281154.1	alanine racemase	-
A2V17_RS11115	2167475..2167834	-	360	WP_000581197.1	holo-ACP synthase	-
A2V17_RS11120	2167838..2168329	-	492	WP_001205912.1	PH domain-containing protein	-
A2V17_RS11125	2168316..2169899	-	1584	WP_001294626.1	PH domain-containing protein	-
A2V17_RS11130	2169892..2170371	-	480	WP_001287087.1	hypothetical protein	-
A2V17_RS11135	2170579..2171139	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T65918 WP_000621175.1 NZ_CP017090:c2166009-2165647 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T65918 NZ_CP017090:c2166009-2165647 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT65918 WP_000948331.1 NZ_CP017090:c2166176-2166006 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT65918 NZ_CP017090:c2166176-2166006 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1