Detailed information of TA system TA065905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 2865952..2866568
• Replicon: chromosome
• Accession: NZ_CP017087
• Organism: Enterobacter sp. HK169

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EnteroDNA1_RS13645
• Protein ID: WP_083239553.1
• Coordinates: 2865952..2866323 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: EnteroDNA1_RS13650
• Protein ID: WP_023309753.1
• Coordinates: 2866326..2866568 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EnteroDNA1_RS13630	2863452..2864354	+	903	WP_010436855.1	formate dehydrogenase subunit beta	-
EnteroDNA1_RS13635	2864351..2864986	+	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
EnteroDNA1_RS13640	2864983..2865912	+	930	WP_023309751.1	formate dehydrogenase accessory protein FdhE	-
EnteroDNA1_RS13645	2865952..2866323	-	372	WP_083239553.1	type II toxin-antitoxin system VapC family toxin	Toxin
EnteroDNA1_RS13650	2866326..2866568	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
EnteroDNA1_RS13655	2866768..2867688	+	921	WP_069303436.1	alpha/beta hydrolase	-
EnteroDNA1_RS13660	2867697..2868638	-	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
EnteroDNA1_RS13665	2868683..2869120	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EnteroDNA1_RS13670	2869117..2869998	-	882	WP_033146965.1	virulence factor BrkB family protein	-
EnteroDNA1_RS13675	2869992..2870591	-	600	WP_047059148.1	glucose-1-phosphatase	-
EnteroDNA1_RS13680	2870680..2871150	-	471	WP_069303437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13716.86 Da
• Isoelectric Point: 6.9790

>T65905 WP_083239553.1 NZ_CP017087:c2866323-2865952 [Enterobacter sp. HK169]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMRLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T65905 NZ_CP017087:c2866323-2865952 [Enterobacter sp. HK169]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAGACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT65905 WP_023309753.1 NZ_CP017087:c2866568-2866326 [Enterobacter sp. HK169]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT65905 NZ_CP017087:c2866568-2866326 [Enterobacter sp. HK169]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTTGACCAGCAAAGTTCATCGG
TTATCCGCGATGCGCTTGGTTTGTGGGGAAATCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0