Detailed information of TA system TA065886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2144548..2145347
• Replicon: chromosome
• Accession: NZ_CP017085
• Organism: Proteus mirabilis strain T18

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: BG029_RS10395
• Protein ID: WP_004247966.1
• Coordinates: 2144823..2145347 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: BG029_RS10390
• Protein ID: WP_004247964.1
• Coordinates: 2144548..2144826 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG029_RS10365	2139898..2140980	+	1083	WP_004242680.1	peptide chain release factor 1	-
BG029_RS10370	2140980..2141828	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
BG029_RS10375	2141806..2142621	+	816	WP_004242688.1	invasion regulator SirB1	-
BG029_RS10380	2142679..2143533	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
BG029_RS10385	2143541..2144470	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
BG029_RS10390	2144548..2144826	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
BG029_RS10395	2144823..2145347	+	525	WP_004247966.1	hypothetical protein	Toxin
BG029_RS10400	2145428..2147128	-	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
BG029_RS10410	2148092..2149033	+	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T65886 WP_004247966.1 NZ_CP017085:2144823-2145347 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T65886 NZ_CP017085:2144823-2145347 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT65886 WP_004247964.1 NZ_CP017085:2144548-2144826 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT65886 NZ_CP017085:2144548-2144826 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2