Detailed information of TA system TA065878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3496571..3497370
• Replicon: chromosome
• Accession: NZ_CP017082
• Organism: Proteus mirabilis strain T21

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: BG257_RS16425
• Protein ID: WP_004247966.1
• Coordinates: 3496571..3497095 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: BG257_RS16430
• Protein ID: WP_004247964.1
• Coordinates: 3497092..3497370 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG257_RS16410	3492885..3493826	-	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-
BG257_RS16420	3494790..3496490	+	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
BG257_RS16425	3496571..3497095	-	525	WP_004247966.1	hypothetical protein	Toxin
BG257_RS16430	3497092..3497370	-	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
BG257_RS16435	3497448..3498377	-	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
BG257_RS16440	3498385..3499239	-	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
BG257_RS16445	3499297..3500112	-	816	WP_004242688.1	invasion regulator SirB1	-
BG257_RS16450	3500090..3500938	-	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
BG257_RS16455	3500938..3502020	-	1083	WP_004242680.1	peptide chain release factor 1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T65878 WP_004247966.1 NZ_CP017082:c3497095-3496571 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T65878 NZ_CP017082:c3497095-3496571 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT65878 WP_004247964.1 NZ_CP017082:c3497370-3497092 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT65878 NZ_CP017082:c3497370-3497092 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2