Detailed information of TA system TA065871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 194799..195687
• Replicon: plasmid pSA3
• Accession: NZ_CP017078
• Organism: Novosphingobium resinovorum strain SA1

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A1D8AGE7
• Locus tag: BES08_RS30385
• Protein ID: WP_069710322.1
• Coordinates: 194799..195323 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1D8AGV2
• Locus tag: BES08_RS30390
• Protein ID: WP_069710391.1
• Coordinates: 195316..195687 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BES08_RS30355	191022..191834	-	813	WP_069710317.1	IS5 family transposase	-
BES08_RS30360	192091..192855	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
BES08_RS30365	193012..193329	-	318	WP_197524555.1	hypothetical protein	-
BES08_RS30370	193353..193616	-	264	WP_069710319.1	hypothetical protein	-
BES08_RS30375	193674..193901	-	228	WP_069710320.1	helix-turn-helix transcriptional regulator	-
BES08_RS30380	193901..194365	-	465	WP_069710321.1	hypothetical protein	-
BES08_RS30385	194799..195323	-	525	WP_069710322.1	RES domain-containing protein	Toxin
BES08_RS30390	195316..195687	-	372	WP_069710391.1	DUF2384 domain-containing protein	Antitoxin
BES08_RS30395	195785..197128	-	1344	WP_069710323.1	replication initiation protein RepC	-
BES08_RS30400	197316..198326	-	1011	WP_069710324.1	plasmid partitioning protein RepB	-
BES08_RS30405	198445..199701	-	1257	WP_083274949.1	plasmid partitioning protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..354886	354886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (12-166)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (17-63)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19370.94 Da
• Isoelectric Point: 5.5636

>T65871 WP_069710322.1 NZ_CP017078:c195323-194799 [Novosphingobium resinovorum]
MPELTDLKPIILWRAYVPRWSHLPLSGEGAARFGGRWNPIGVHTIYAARELSTAWAEYNQGFVQHPALIAQLELTGARLV
DTTDPVVLDEWGFDQDIHKTEWRSELDKGAVAATHAAYQMLFDAKVDGVIYPSFMSPGGSCVALWRWNQSDGPVLKVVDP
DGRLPTTSASWRTP

Nucleotide

• Length: 525 bp

>T65871 NZ_CP017078:c195323-194799 [Novosphingobium resinovorum]
ATGCCTGAGTTGACAGACCTTAAGCCGATCATTTTGTGGCGAGCCTACGTTCCTCGCTGGTCACATCTACCGCTATCGGG
GGAGGGCGCTGCTCGTTTTGGAGGCCGTTGGAATCCGATCGGCGTCCACACGATTTACGCCGCGCGGGAATTGTCGACCG
CCTGGGCAGAATACAACCAAGGGTTCGTGCAGCATCCAGCATTGATCGCTCAACTCGAGCTGACCGGCGCCCGCCTTGTC
GACACGACCGATCCCGTAGTTCTCGACGAATGGGGCTTCGACCAGGACATTCACAAGACGGAGTGGCGGTCGGAATTGGA
CAAGGGGGCTGTCGCTGCCACGCATGCCGCATATCAAATGCTTTTTGACGCGAAGGTCGATGGGGTCATCTATCCCTCTT
TTATGTCTCCAGGTGGAAGCTGTGTCGCACTCTGGCGCTGGAACCAGTCGGATGGTCCGGTTCTAAAGGTTGTTGACCCT
GACGGTCGGCTTCCAACAACCTCGGCGTCTTGGCGAACGCCATAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13008.96 Da
• Isoelectric Point: 8.4934

>AT65871 WP_069710391.1 NZ_CP017078:c195687-195316 [Novosphingobium resinovorum]
MQPQGLEIGAVRFGDGGSPFLSAPRLSEQLGVTQSELAKLVGIARNTLTAKTATRKVDAALSPIVRILAMAAEMAGDENR
AVIWFKHQPIPGWAGKTAYDLVGDGKADQVLAYLEAVRSGVYA

Nucleotide

• Length: 372 bp

>AT65871 NZ_CP017078:c195687-195316 [Novosphingobium resinovorum]
ATGCAGCCCCAGGGTCTCGAGATAGGTGCAGTTCGGTTTGGCGATGGCGGCTCGCCCTTCCTGTCGGCGCCGCGGCTGTC
CGAACAGCTCGGCGTGACACAGTCGGAGCTGGCCAAGCTTGTCGGAATTGCCCGAAATACGCTCACCGCCAAAACCGCCA
CCCGGAAGGTGGACGCGGCGCTAAGCCCGATCGTGCGTATCCTGGCTATGGCCGCAGAGATGGCTGGCGACGAAAATCGT
GCGGTAATCTGGTTTAAGCACCAACCAATTCCTGGCTGGGCGGGCAAGACGGCTTACGATCTGGTGGGAGACGGCAAGGC
AGATCAGGTCTTGGCCTATCTGGAGGCTGTGCGCTCGGGCGTCTATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1D8AGE7

Antitoxin

• AlphaFold DB: A0A1D8AGV2