Detailed information of TA system TA065870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 103589..104186
• Replicon: plasmid pSA3
• Accession: NZ_CP017078
• Organism: Novosphingobium resinovorum strain SA1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: BES08_RS32440
• Protein ID: WP_008832692.1
• Coordinates: 103589..103885 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1D8AG74
• Locus tag: BES08_RS29955
• Protein ID: WP_069710292.1
• Coordinates: 103890..104186 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BES08_RS29930	99442..100656	+	1215	WP_069710290.1	type IV secretory system conjugative DNA transfer family protein	-
BES08_RS29935	100653..101897	+	1245	WP_069710291.1	hypothetical protein	-
BES08_RS29940	101939..102166	+	228	WP_008832689.1	hypothetical protein	-
BES08_RS29945	102141..103130	+	990	WP_008832690.1	hypothetical protein	-
BES08_RS29950	103148..103516	+	369	WP_008832691.1	DUF3768 domain-containing protein	-
BES08_RS32440	103589..103885	+	297	WP_008832692.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BES08_RS29955	103890..104186	+	297	WP_069710292.1	putative addiction module antidote protein	Antitoxin
BES08_RS29960	104567..105421	-	855	WP_036528669.1	Abi family protein	-
BES08_RS29965	105896..106090	+	195	WP_036528671.1	hypothetical protein	-
BES08_RS29970	106095..106559	-	465	WP_036528673.1	hypothetical protein	-
BES08_RS29975	106920..107231	-	312	WP_197524564.1	integration host factor subunit alpha	-
BES08_RS29980	108119..108565	+	447	WP_051587082.1	DUF736 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..354886	354886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10852.56 Da
• Isoelectric Point: 10.3136

>T65870 WP_008832692.1 NZ_CP017078:103589-103885 [Novosphingobium resinovorum]
MIEMIQSNTFNQWLAGLRDSNAKARIQVRLRRLSLGNAGDVKPVGDGISELRIDYGPGYRVYYMKRGPILIVLLCGGDKS
SQARDIAQAKSIAAAWKE

Nucleotide

• Length: 297 bp

>T65870 NZ_CP017078:103589-103885 [Novosphingobium resinovorum]
ATGATCGAGATGATCCAGTCTAACACCTTCAACCAATGGTTGGCTGGTTTGCGCGATTCAAATGCGAAAGCCCGGATACA
AGTCCGTCTGCGTAGATTGAGCCTTGGAAATGCAGGCGATGTTAAACCCGTAGGGGACGGAATATCCGAACTTCGTATCG
ATTATGGGCCGGGCTATCGCGTGTATTATATGAAGCGCGGGCCAATCCTAATTGTCCTGCTTTGTGGTGGTGACAAGAGC
AGTCAGGCTCGCGACATAGCGCAGGCCAAATCAATTGCGGCCGCATGGAAGGAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10349.62 Da
• Isoelectric Point: 4.5258

>AT65870 WP_069710292.1 NZ_CP017078:103890-104186 [Novosphingobium resinovorum]
MDVTFSRYDPADFLKTEEDIAAYLQAAAEDGDTALIAAALGDVVRARNISKLSRDTGLSREGIYKALSGEGNPSFATIGK
IAGALGLRISFQPNQTAI

Nucleotide

• Length: 297 bp

>AT65870 NZ_CP017078:103890-104186 [Novosphingobium resinovorum]
ATGGACGTGACTTTTAGCCGTTACGATCCCGCCGACTTCCTCAAGACTGAAGAGGACATTGCGGCATATCTGCAAGCCGC
TGCGGAAGATGGCGATACCGCTTTGATCGCAGCAGCTCTTGGGGACGTGGTACGTGCTCGCAATATTAGCAAGCTGTCCC
GCGATACCGGGCTTAGTCGGGAGGGTATCTACAAGGCCCTGTCGGGTGAAGGAAATCCGAGTTTCGCGACTATCGGCAAG
ATTGCCGGCGCGTTGGGGCTGCGCATCAGTTTCCAACCCAACCAAACGGCGATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D8AG74