Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5752951..5754680 Replicon chromosome
Accession NZ_CP017073
Organism Pseudomonas putida strain PP112420

Toxin (Protein)


Gene name pmenT Uniprot ID -
Locus tag BG030_RS26315 Protein ID WP_071984741.1
Coordinates 5754039..5754680 (-) Length 214 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID -
Locus tag BG030_RS26310 Protein ID WP_060493824.1
Coordinates 5752951..5754039 (-) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG030_RS26295 5748579..5749397 - 819 WP_171577246.1 HDOD domain-containing protein -
BG030_RS26300 5749580..5750776 + 1197 WP_071984740.1 class I SAM-dependent rRNA methyltransferase -
BG030_RS26305 5751048..5752889 + 1842 WP_012274676.1 dihydroxy-acid dehydratase -
BG030_RS26310 5752951..5754039 - 1089 WP_060493824.1 macro domain-containing protein Antitoxin
BG030_RS26315 5754039..5754680 - 642 WP_071984741.1 DUF4433 domain-containing protein Toxin
BG030_RS26320 5754677..5755255 - 579 WP_081366137.1 DUF4433 domain-containing protein -
BG030_RS26325 5755352..5756407 - 1056 WP_060493822.1 phosphorylcholine phosphatase -
BG030_RS26330 5756652..5758043 + 1392 WP_060493821.1 L-cystine transporter -
BG030_RS26335 5758079..5758594 - 516 WP_012274680.1 dihydrofolate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 214 a.a.        Molecular weight: 24643.24 Da        Isoelectric Point: 9.3544

>T65867 WP_071984741.1 NZ_CP017073:c5754680-5754039 [Pseudomonas putida]
MIYSSLNPSKALIWRIVHRDNLPWLLDHGLHAVSTGKLSSAYVGIGNPELISKRAQRHVPIEPNGTLADYVPFYFTPFSV
MMKNIHSGWGVSQRRNEEIVILVSSLYHAQSLGLRFIFTDAHAYPHWAGYYSDLTQLDKIDWEVLQRRDFKRDPDDPRKM
ERYQAEALIHGHVPVAGLLGIVCYNEALKRRIDKEVQQRQLNLAVYARPGWYF

Download         Length: 642 bp

>T65867 NZ_CP017073:c5754680-5754039 [Pseudomonas putida]
ATGATCTATTCCAGTCTCAACCCATCCAAGGCGCTCATCTGGCGAATTGTTCACCGCGATAACCTGCCGTGGCTTCTGGA
TCATGGGCTTCACGCTGTCTCCACCGGGAAGCTATCCAGTGCTTACGTTGGAATCGGGAACCCAGAGCTGATCAGCAAGC
GAGCGCAACGCCACGTGCCCATAGAGCCCAATGGGACGCTGGCAGATTATGTGCCGTTTTACTTCACCCCATTCTCCGTG
ATGATGAAGAACATCCATTCGGGGTGGGGCGTTTCTCAGCGGCGGAATGAAGAGATCGTGATTCTTGTCTCCAGCCTGTA
CCATGCGCAGTCGCTGGGTCTGCGGTTTATCTTCACGGACGCACATGCCTACCCGCATTGGGCCGGTTACTACAGCGACC
TTACGCAGCTCGACAAAATAGATTGGGAGGTTTTGCAGCGCAGGGACTTCAAGCGTGACCCTGATGACCCCCGAAAAATG
GAGCGCTATCAGGCTGAGGCCCTTATCCATGGACATGTCCCGGTCGCTGGCCTTCTGGGAATAGTCTGCTACAACGAAGC
TTTGAAGCGTCGTATCGACAAGGAGGTTCAGCAACGTCAGCTGAATCTTGCCGTATATGCTCGCCCTGGGTGGTACTTCT
GA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40462.47 Da        Isoelectric Point: 5.8983

>AT65867 WP_060493824.1 NZ_CP017073:c5754039-5752951 [Pseudomonas putida]
MIRFMQGNLLEAKAEALVNTVNTVGVMGKGIALMFKERFAENYRLYAAACKAKEVVTGKMFVTEVRELDGPRWIVNFPTK
RHWRSPSQIGWIVDGLHDLRNFILENQVASIAVPPLGAGNGGLPWAAVREQIESVLGDLDADILVFEPTANYQNVSKRSG
VENLTPARALIAELVRRYWVLGMECSLLEVQKLAWFLERSIERFTPGSNPLELKFVAHKYGPYANRLEHLLDNLDGSYLH
CGKRISDAGPLDVIWFDEGRKSFVQAYLNSEAKAYLPALEFTAELIDGFESPFGMELLATVDWLLHEEGVEPTIRAIREH
LKSWSGGAGAAARKSKLFDDESLGIALQKLSMFQTDPVTSLH

Download         Length: 1089 bp

>AT65867 NZ_CP017073:c5754039-5752951 [Pseudomonas putida]
ATGATTCGCTTTATGCAAGGTAACCTGCTGGAGGCAAAAGCCGAAGCGCTGGTTAATACAGTCAACACAGTAGGGGTGAT
GGGGAAAGGCATTGCGCTGATGTTCAAGGAGCGTTTTGCCGAAAACTATCGCCTGTATGCTGCTGCCTGCAAAGCGAAAG
AAGTCGTTACCGGGAAGATGTTTGTTACTGAGGTGCGTGAGCTGGATGGTCCGCGCTGGATTGTGAATTTTCCCACCAAG
CGGCATTGGCGCTCACCTTCGCAAATCGGCTGGATCGTCGATGGTCTCCATGATTTGCGGAACTTCATCCTCGAGAACCA
AGTAGCATCGATTGCAGTCCCGCCACTTGGCGCTGGCAACGGAGGGTTGCCTTGGGCTGCGGTACGAGAACAGATTGAGA
GTGTTCTAGGTGATCTGGATGCGGATATTCTCGTTTTCGAGCCTACGGCAAATTATCAGAACGTTTCCAAGCGCAGTGGT
GTTGAGAATTTAACCCCGGCTCGAGCCTTGATCGCAGAGCTGGTGCGCCGCTACTGGGTGCTGGGAATGGAGTGCAGCTT
GCTCGAAGTCCAGAAGCTTGCCTGGTTTCTGGAGCGATCAATCGAGCGGTTCACGCCAGGTTCCAACCCATTGGAGTTGA
AGTTTGTCGCTCATAAGTATGGTCCTTACGCCAACCGGCTTGAACATCTGCTAGATAATCTGGATGGCAGCTATTTGCAC
TGCGGAAAGCGGATCAGCGATGCAGGCCCTCTGGACGTGATCTGGTTTGATGAGGGGCGCAAGTCATTTGTACAGGCGTA
TTTGAACAGTGAAGCCAAGGCCTATTTGCCGGCGTTGGAGTTCACTGCCGAATTGATTGATGGTTTCGAGTCTCCGTTTG
GTATGGAGCTACTGGCCACCGTTGATTGGCTATTACATGAAGAGGGTGTTGAGCCGACGATTCGCGCAATTCGCGAACAT
CTGAAAAGTTGGTCTGGAGGGGCAGGTGCCGCTGCTCGAAAAAGCAAATTGTTCGATGACGAGTCACTTGGAATCGCTCT
CCAAAAGCTGTCAATGTTTCAAACGGACCCCGTCACCTCGCTTCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References