Detailed information of TA system TA065862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3753655..3754291
• Replicon: chromosome
• Accession: NZ_CP017072
• Organism: Bacillus sp. FJAT-14266

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BGM23_RS19495
• Protein ID: WP_003156187.1
• Coordinates: 3753655..3754005 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: BGM23_RS19500
• Protein ID: WP_003225183.1
• Coordinates: 3754010..3754291 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGM23_RS19455	3748699..3749298	-	600	WP_003234303.1	phosphoserine phosphatase RsbX	-
BGM23_RS19460	3749298..3750086	-	789	WP_029727187.1	RNA polymerase sigma factor SigB	-
BGM23_RS19465	3750052..3750534	-	483	WP_003234299.1	anti-sigma B factor RsbW	-
BGM23_RS19470	3750531..3750860	-	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
BGM23_RS19475	3750922..3751929	-	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
BGM23_RS19480	3751941..3752342	-	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
BGM23_RS19485	3752346..3752711	-	366	WP_003225190.1	RsbT antagonist protein RsbS	-
BGM23_RS19490	3752716..3753540	-	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
BGM23_RS19495	3753655..3754005	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BGM23_RS19500	3754010..3754291	-	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BGM23_RS19505	3754407..3755576	-	1170	WP_029727188.1	alanine racemase	-
BGM23_RS19510	3755690..3756706	-	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
BGM23_RS19515	3756872..3757237	-	366	WP_015252768.1	holo-ACP synthase	-
BGM23_RS19520	3757332..3757931	+	600	WP_003246687.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T65862 WP_003156187.1 NZ_CP017072:c3754005-3753655 [Bacillus sp. FJAT-14266]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T65862 NZ_CP017072:c3754005-3753655 [Bacillus sp. FJAT-14266]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT65862 WP_003225183.1 NZ_CP017072:c3754291-3754010 [Bacillus sp. FJAT-14266]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT65862 NZ_CP017072:c3754291-3754010 [Bacillus sp. FJAT-14266]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32