Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1643922..1644520 Replicon chromosome
Accession NZ_CP017071
Organism Geobacillus thermoleovorans strain FJAT-2391

Toxin (Protein)


Gene name mazF Uniprot ID G8N5V4
Locus tag BGM21_RS08200 Protein ID WP_011231143.1
Coordinates 1644191..1644520 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag BGM21_RS08195 Protein ID WP_011231142.1
Coordinates 1643922..1644191 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGM21_RS08150 1639188..1639487 + 300 WP_069304229.1 transporter suffix domain-containing protein -
BGM21_RS08155 1639497..1640294 + 798 WP_011231137.1 SGNH/GDSL hydrolase family protein -
BGM21_RS08160 1640291..1641205 + 915 WP_011231138.1 ABC transporter ATP-binding protein -
BGM21_RS08165 1641198..1642178 + 981 WP_011231139.1 ABC transporter permease -
BGM21_RS08170 1642247..1642714 - 468 WP_021321939.1 hypothetical protein -
BGM21_RS08175 1642728..1643312 - 585 Protein_1584 hypothetical protein -
BGM21_RS08195 1643922..1644191 + 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BGM21_RS08200 1644191..1644520 + 330 WP_011231143.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BGM21_RS08205 1644973..1645806 + 834 WP_011231146.1 pirin family protein -
BGM21_RS08210 1645902..1646651 + 750 WP_021321363.1 oxygen-insensitive NADPH nitroreductase -
BGM21_RS08215 1646841..1647584 + 744 WP_021321364.1 NAD-dependent protein deacylase -
BGM21_RS08220 1647765..1648943 + 1179 WP_047757857.1 acyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12071.86 Da        Isoelectric Point: 7.1331

>T65860 WP_011231143.1 NZ_CP017071:1644191-1644520 [Geobacillus thermoleovorans]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Download         Length: 330 bp

>T65860 NZ_CP017071:1644191-1644520 [Geobacillus thermoleovorans]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT65860 WP_011231142.1 NZ_CP017071:1643922-1644191 [Geobacillus thermoleovorans]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT65860 NZ_CP017071:1643922-1644191 [Geobacillus thermoleovorans]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACCGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9EUB7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References