Detailed information of TA system TA065854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-yefM/YoeB-RelB
• Location: 2712450..2712975
• Replicon: chromosome
• Accession: NZ_CP017065
• Organism: Lacticaseibacillus casei strain LC5

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: -
• Locus tag: BGL52_RS13465
• Protein ID: WP_087912902.1
• Coordinates: 2712450..2712707 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: BGL52_RS13470
• Protein ID: WP_087912903.1
• Coordinates: 2712700..2712975 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGL52_RS13445	2709329..2710153	+	825	WP_087912899.1	LysR family transcriptional regulator	-
BGL52_RS13450	2710178..2711149	-	972	WP_087912900.1	TDT family transporter	-
BGL52_RS13455	2711307..2711954	-	648	WP_087912901.1	CPBP family intramembrane metalloprotease	-
BGL52_RS13465	2712450..2712707	-	258	WP_087912902.1	Txe/YoeB family addiction module toxin	Toxin
BGL52_RS13470	2712700..2712975	-	276	WP_087912903.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BGL52_RS13475	2713144..2714745	-	1602	WP_087912904.1	ABC transporter ATP-binding protein/permease	-
BGL52_RS13480	2714950..2715594	-	645	WP_087912905.1	HD domain-containing protein	-
BGL52_RS13485	2715731..2716147	+	417	WP_087912906.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10161.59 Da
• Isoelectric Point: 8.0783

>T65854 WP_087912902.1 NZ_CP017065:c2712707-2712450 [Lacticaseibacillus casei]
MIKRWTDDAWHDYISWFEQGDKATVRKINRLIASIDRTPFDGLGKPEPLKHALSGMWSRRITQEHRLIYEIETNTMTIIS
CRDHY

Nucleotide

• Length: 258 bp

>T65854 NZ_CP017065:c2712707-2712450 [Lacticaseibacillus casei]
ATGATTAAGCGCTGGACTGACGATGCCTGGCACGACTACATCAGTTGGTTTGAGCAAGGTGATAAAGCTACGGTCAGAAA
AATCAATCGACTCATCGCGTCGATTGATCGGACACCGTTTGATGGCCTGGGTAAACCAGAACCGTTGAAACACGCCTTGT
CTGGCATGTGGTCGCGTCGAATCACGCAAGAACACCGCTTAATTTACGAAATTGAGACAAACACAATGACAATCATTTCC
TGTCGCGATCATTACTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10163.49 Da
• Isoelectric Point: 7.2020

>AT65854 WP_087912903.1 NZ_CP017065:c2712975-2712700 [Lacticaseibacillus casei]
MDAVSYSVFRRNLKHYLQAVNDDATPLLITSRDNAADTAVLMNKRDFDAMQETMRILSNPELMAKIRRGQAQIAAGKTRQ
HDLKDVGGGDD

Nucleotide

• Length: 276 bp

>AT65854 NZ_CP017065:c2712975-2712700 [Lacticaseibacillus casei]
ATGGACGCTGTTTCCTACTCAGTTTTCCGCCGCAATCTCAAGCACTACCTTCAAGCAGTCAATGATGACGCTACGCCACT
CTTGATTACTAGCCGTGATAATGCGGCTGATACTGCAGTCTTGATGAATAAACGTGATTTTGATGCCATGCAAGAAACGA
TGAGGATCTTATCGAATCCTGAACTCATGGCCAAGATCCGCCGTGGCCAAGCTCAGATTGCCGCTGGTAAAACCCGGCAG
CATGATTTAAAAGATGTCGGTGGCGGGGATGATTAA