65853

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2628868..2629518 Replicon chromosome
Accession NZ_CP017065
Organism Lacticaseibacillus casei strain LC5

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag BGL52_RS13040 Protein ID WP_005686631.1
Coordinates 2628868..2629251 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0R1EMB2
Locus tag BGL52_RS13045 Protein ID WP_010491246.1
Coordinates 2629270..2629518 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGL52_RS13020 2624219..2625226 + 1008 WP_003662706.1 L-lactate dehydrogenase -
BGL52_RS13025 2625534..2626904 + 1371 WP_075761421.1 peptidoglycan binding domain-containing protein -
BGL52_RS13030 2627112..2627777 + 666 WP_010491258.1 CBS domain-containing protein -
BGL52_RS13035 2628100..2628798 + 699 WP_087912844.1 L,D-transpeptidase -
BGL52_RS13040 2628868..2629251 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BGL52_RS13045 2629270..2629518 - 249 WP_010491246.1 antitoxin Antitoxin
BGL52_RS13050 2629617..2630756 - 1140 WP_087912845.1 alanine racemase -
BGL52_RS13055 2630743..2631117 - 375 WP_010491243.1 holo-ACP synthase -
BGL52_RS13060 2631363..2632874 - 1512 WP_087912846.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T65853 WP_005686631.1 NZ_CP017065:c2629251-2628868 [Lacticaseibacillus casei]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T65853 NZ_CP017065:c2629251-2628868 [Lacticaseibacillus casei]
ATGGATGTAACCGTAAAACGCGGAGATGTATTCTTCGCCGATTTATCGCCGGTTGTCGGATCTGAACAGGGTGGCAATCG
ACCTGTACTTATTATTCAAAACAACGTTGGCAACCACTATAGTCCTACGGTTATTGTGGCGGCCATTACCTCCAAGATCC
AAAAACCCAAGATGCCCACTCATGTTGGCTTACGGGCAAAACAAGATGGGGTTGAACGTAACTCGGTGATTTTGCTAGAA
CAGATTCGCACCATCGATAAGCAGCGATTACAGGCGCGCGTGACAGCTTTATCAAGCGCAAAAATGGCGGCCGTTGATCG
TGCGTTGGCAATTTCAGTAGGGCTCGTTAGTTTGCCCAAACCAAAAACATACAATAAAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8971.07 Da        Isoelectric Point: 4.6061

>AT65853 WP_010491246.1 NZ_CP017065:c2629518-2629270 [Lacticaseibacillus casei]
MKKQTSVTVTFDAAIKARLDAYCELNDTDTQDVITTAVAKFLQSQAPDINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT65853 NZ_CP017065:c2629518-2629270 [Lacticaseibacillus casei]
ATGAAGAAGCAAACGTCAGTAACGGTAACGTTTGATGCTGCTATTAAGGCGCGGTTAGATGCCTATTGTGAGTTGAATGA
CACGGATACTCAAGATGTGATCACGACAGCAGTGGCCAAATTTTTGCAGTCACAAGCACCCGATATCAACCAGCTGATTT
CCGGCTACGTTGCCATGGGACAGATCAATACAGAGATCAGTCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB A0A0R1EMB2

References