Detailed information of TA system TA065851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2302847..2303825
• Replicon: chromosome
• Accession: NZ_CP017060
• Organism: Bacillus cereus strain FORC_047

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: FORC47_RS12140
• Protein ID: WP_089171000.1
• Coordinates: 2302847..2303584 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: FORC47_RS12145
• Protein ID: WP_000237818.1
• Coordinates: 2303697..2303825 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC47_RS12115	2298489..2299271	+	783	WP_089170998.1	class I SAM-dependent methyltransferase	-
FORC47_RS12120	2299429..2301105	-	1677	WP_157676081.1	alpha-keto acid decarboxylase family protein	-
FORC47_RS12125	2301222..2301704	+	483	WP_053564493.1	MarR family winged helix-turn-helix transcriptional regulator	-
FORC47_RS12130	2301871..2302608	+	738	WP_016512840.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FORC47_RS12140	2302847..2303584	+	738	WP_089171000.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FORC47_RS12145	2303697..2303825	+	129	WP_000237818.1	hypothetical protein	Antitoxin
FORC47_RS12150	2303898..2304074	+	177	WP_088024060.1	stage II sporulation protein SB	-
FORC47_RS12155	2304095..2304484	-	390	WP_089171001.1	YxeA family protein	-
FORC47_RS12160	2304696..2306165	+	1470	WP_089171002.1	beta-Ala-His dipeptidase	-
FORC47_RS12165	2306411..2307220	+	810	WP_089171003.1	C39 family peptidase	-
FORC47_RS12170	2307246..2307848	+	603	WP_088115306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28341.06 Da
• Isoelectric Point: 8.2998

>T65851 WP_089171000.1 NZ_CP017060:2302847-2303584 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHMEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T65851 NZ_CP017060:2302847-2303584 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATGGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT65851 WP_000237818.1 NZ_CP017060:2303697-2303825 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT65851 NZ_CP017060:2303697-2303825 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5