Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 126988..127631 Replicon plasmid unnamed2
Accession NZ_CP017059
Organism Citrobacter freundii strain SL151

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag BFQ28_RS26175 Protein ID WP_008322233.1
Coordinates 127215..127631 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BFQ28_RS26170 Protein ID WP_001261276.1
Coordinates 126988..127218 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFQ28_RS26140 122925..123275 - 351 WP_004189161.1 IS66 family insertion sequence element accessory protein TnpB -
BFQ28_RS26145 123272..123712 - 441 WP_004189163.1 IS66 family insertion sequence hypothetical protein -
BFQ28_RS26150 123795..123977 - 183 Protein_134 transposase -
BFQ28_RS26155 124096..124596 - 501 WP_001752509.1 non-heme ferritin-like protein -
BFQ28_RS26160 124923..125627 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
BFQ28_RS26165 125713..126735 + 1023 WP_008322227.1 helicase UvrD -
BFQ28_RS26170 126988..127218 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BFQ28_RS26175 127215..127631 + 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
BFQ28_RS28320 127825..127994 + 170 Protein_140 DUF596 domain-containing protein -
BFQ28_RS26180 128177..129097 - 921 WP_008322235.1 carbohydrate kinase -
BFQ28_RS26185 129094..130464 - 1371 WP_008322237.1 hypothetical protein -
BFQ28_RS26190 130542..131561 - 1020 WP_004113181.1 ribose ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / ARR-3 / sul1 / qnrB6 / qacE / mph(A) / aac(3)-IId / blaTEM-1B / tet(D) / catA2 / sul2 / dfrA27 / aadA16 - 1..171233 171233


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T65850 WP_008322233.1 NZ_CP017059:127215-127631 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T65850 NZ_CP017059:127215-127631 [Citrobacter freundii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT65850 WP_001261276.1 NZ_CP017059:126988-127218 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT65850 NZ_CP017059:126988-127218 [Citrobacter freundii]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References