Detailed information of TA system TA065849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 99549..100352
• Replicon: plasmid unnamed2
• Accession: NZ_CP017059
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A7W3E3C9
• Locus tag: BFQ28_RS26015
• Protein ID: WP_022652313.1
• Coordinates: 99549..100079 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: BFQ28_RS26020
• Protein ID: WP_022652312.1
• Coordinates: 100083..100352 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS25990	95435..95782	-	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-
BFQ28_RS25995	95779..96162	-	384	WP_001201739.1	IS66 family insertion sequence hypothetical protein	-
BFQ28_RS26000	96271..97422	-	1152	WP_022652315.1	IS30-like element IS30 family transposase	-
BFQ28_RS26005	97487..98383	+	897	Protein_106	IS3 family transposase	-
BFQ28_RS26010	98524..99417	-	894	WP_077873527.1	IS5 family transposase	-
BFQ28_RS26015	99549..100079	-	531	WP_022652313.1	GNAT family N-acetyltransferase	Toxin
BFQ28_RS26020	100083..100352	-	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
BFQ28_RS26025	101208..102197	+	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
BFQ28_RS26030	102523..103263	-	741	WP_022652310.1	site-specific integrase	-
BFQ28_RS26035	103740..104047	+	308	Protein_112	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib-cr / ARR-3 / sul1 / qnrB6 / qacE / mph(A) / aac(3)-IId / blaTEM-1B / tet(D) / catA2 / sul2 / dfrA27 / aadA16	-	1..171233	171233
	-	inside	IScluster/Tn	-	-	91980..105361	13381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20349.26 Da
• Isoelectric Point: 6.7496

>T65849 WP_022652313.1 NZ_CP017059:c100079-99549 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T65849 NZ_CP017059:c100079-99549 [Citrobacter freundii]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT65849 WP_022652312.1 NZ_CP017059:c100352-100083 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT65849 NZ_CP017059:c100352-100083 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3E3C9

Antitoxin

• AlphaFold DB: A0A2Z3XDS5