Detailed information of TA system TA065847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 30925..31411
• Replicon: plasmid pPS2
• Accession: NZ_CP017056
• Organism: Providencia stuartii strain BE2467

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: -
• Locus tag: BGK56_RS22030
• Protein ID: WP_012368911.1
• Coordinates: 31145..31411 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: B1VJ89
• Locus tag: BGK56_RS22025
• Protein ID: WP_012368910.1
• Coordinates: 30925..31155 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGK56_RS21975	25984..26340	-	357	WP_012368898.1	hypothetical protein	-
BGK56_RS21980	26350..27519	-	1170	WP_012368899.1	relaxase/mobilization nuclease domain-containing protein	-
BGK56_RS21985	27509..28078	-	570	WP_020803314.1	TraY domain-containing protein	-
BGK56_RS22000	28787..29071	-	285	WP_012368902.1	putative addiction module antidote protein	-
BGK56_RS22005	29073..29369	-	297	WP_012368903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BGK56_RS22010	29701..30045	-	345	WP_012368905.1	hypothetical protein	-
BGK56_RS22895	30206..30367	-	162	WP_012368907.1	hypothetical protein	-
BGK56_RS22015	30372..30554	-	183	WP_012368908.1	hypothetical protein	-
BGK56_RS22025	30925..31155	-	231	WP_012368910.1	transcriptional regulator	Antitoxin
BGK56_RS22030	31145..31411	-	267	WP_012368911.1	hypothetical protein	Toxin
BGK56_RS22035	31433..31984	-	552	WP_020803329.1	hypothetical protein	-
BGK56_RS22040	32054..32260	-	207	WP_012368913.1	hypothetical protein	-
BGK56_RS22045	32315..32839	-	525	WP_012368914.1	J domain-containing protein	-
BGK56_RS22050	33830..34684	+	855	WP_020803325.1	replication initiation protein	-
BGK56_RS22530	34700..35089	+	390	WP_012368861.1	hypothetical protein	-
BGK56_RS22060	35192..35662	+	471	WP_050955858.1	hypothetical protein	-
BGK56_RS22065	35652..35858	+	207	WP_041707207.1	hypothetical protein	-
BGK56_RS22070	35931..36197	+	267	WP_012368864.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38206	38206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-35)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10171.70 Da
• Isoelectric Point: 10.1699

>T65847 WP_012368911.1 NZ_CP017056:c31411-31145 [Providencia stuartii]
MKTIRKNKGDVTYYLSRENNDSYRLIKKIKARATHLVKDGHKTTKVTLSDLLLTHDQLYNLDYSLNGLRADDKATIELLI
GEFFKNGK

Nucleotide

• Length: 267 bp

>T65847 NZ_CP017056:c31411-31145 [Providencia stuartii]
ATGAAAACCATAAGAAAAAACAAAGGTGATGTAACTTATTATTTAAGCAGAGAGAATAACGATAGTTATCGTCTTATTAA
AAAAATCAAGGCGAGAGCGACTCATTTAGTTAAAGACGGGCATAAAACAACCAAAGTAACATTAAGTGATTTGTTATTAA
CGCATGACCAACTTTACAACTTAGATTATTCATTAAACGGGCTGAGGGCAGATGATAAAGCCACAATTGAATTGCTTATA
GGGGAGTTTTTTAAAAATGGAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8786.98 Da
• Isoelectric Point: 8.5162

>AT65847 WP_012368910.1 NZ_CP017056:c31155-30925 [Providencia stuartii]
MENNAKNIKALRLKTGLTQKEMAERYGVGIRTWQKKEEDGTQSSQKLSNFDFEYLLLLAGEHPDFVLEKRNKEIAS

Nucleotide

• Length: 231 bp

>AT65847 NZ_CP017056:c31155-30925 [Providencia stuartii]
ATGGAAAATAATGCAAAAAATATAAAAGCGTTGAGATTAAAAACAGGTTTAACACAAAAGGAAATGGCAGAAAGGTACGG
GGTAGGGATCAGAACTTGGCAAAAAAAAGAGGAGGACGGGACGCAAAGCAGTCAGAAGTTATCAAATTTTGATTTTGAAT
ACTTGCTGTTATTGGCTGGTGAGCATCCTGATTTTGTGCTGGAAAAAAGAAATAAGGAAATTGCAAGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1I3XBZ7