Detailed information of TA system TA065846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 28787..29369
• Replicon: plasmid pPS2
• Accession: NZ_CP017056
• Organism: Providencia stuartii strain BE2467

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B1VJ82
• Locus tag: BGK56_RS22005
• Protein ID: WP_012368903.1
• Coordinates: 29073..29369 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B1VJ81
• Locus tag: BGK56_RS22000
• Protein ID: WP_012368902.1
• Coordinates: 28787..29071 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGK56_RS21960	24396..25058	+	663	WP_012368895.1	plasmid stability/partitioning protein	-
BGK56_RS21965	25132..25410	+	279	WP_012368896.1	hypothetical protein	-
BGK56_RS21970	25480..26007	+	528	WP_012368897.1	restriction endonuclease	-
BGK56_RS21975	25984..26340	-	357	WP_012368898.1	hypothetical protein	-
BGK56_RS21980	26350..27519	-	1170	WP_012368899.1	relaxase/mobilization nuclease domain-containing protein	-
BGK56_RS21985	27509..28078	-	570	WP_020803314.1	TraY domain-containing protein	-
BGK56_RS22000	28787..29071	-	285	WP_012368902.1	putative addiction module antidote protein	Antitoxin
BGK56_RS22005	29073..29369	-	297	WP_012368903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BGK56_RS22010	29701..30045	-	345	WP_012368905.1	hypothetical protein	-
BGK56_RS22895	30206..30367	-	162	WP_012368907.1	hypothetical protein	-
BGK56_RS22015	30372..30554	-	183	WP_012368908.1	hypothetical protein	-
BGK56_RS22025	30925..31155	-	231	WP_012368910.1	transcriptional regulator	-
BGK56_RS22030	31145..31411	-	267	WP_012368911.1	hypothetical protein	-
BGK56_RS22035	31433..31984	-	552	WP_020803329.1	hypothetical protein	-
BGK56_RS22040	32054..32260	-	207	WP_012368913.1	hypothetical protein	-
BGK56_RS22045	32315..32839	-	525	WP_012368914.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38206	38206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11325.21 Da
• Isoelectric Point: 10.2910

>T65846 WP_012368903.1 NZ_CP017056:c29369-29073 [Providencia stuartii]
MIEIRQTEEVKKWLKGLKDKIAKAKILTRIERMKEGNYGDVEPIGDGYSELRIHQGKGYRVYFANRNNEIILLLCGGDKT
TQQADIKKAKQIAKKWGF

Nucleotide

• Length: 297 bp

>T65846 NZ_CP017056:c29369-29073 [Providencia stuartii]
ATGATTGAAATCAGGCAAACAGAAGAAGTTAAAAAATGGCTTAAAGGATTAAAAGATAAAATAGCCAAAGCAAAAATTTT
AACACGGATTGAGCGGATGAAAGAGGGAAATTATGGAGATGTTGAGCCGATAGGTGATGGATATTCAGAGTTAAGAATTC
ATCAGGGCAAGGGTTACAGAGTTTATTTTGCCAACCGAAACAATGAAATTATTTTATTGCTTTGTGGTGGTGATAAAACA
ACACAACAAGCAGACATAAAAAAAGCAAAACAGATAGCGAAAAAATGGGGGTTCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10302.61 Da
• Isoelectric Point: 4.6286

>AT65846 WP_012368902.1 NZ_CP017056:c29071-28787 [Providencia stuartii]
MKTKPELFDVVDYLEDEEDIQAYLNAALDEDDESYFLAALGNIARARNISQLSKETGMSREGIYKALSGTGNPSFKTISK
ISKALGLKIHFAGA

Nucleotide

• Length: 285 bp

>AT65846 NZ_CP017056:c29071-28787 [Providencia stuartii]
ATGAAAACAAAACCAGAATTATTTGATGTAGTCGATTACCTAGAGGATGAAGAAGATATTCAGGCATATCTCAATGCCGC
TCTGGATGAAGATGATGAAAGCTACTTTTTAGCAGCACTGGGAAATATTGCCAGAGCAAGAAATATCAGCCAACTTTCAA
AAGAAACAGGAATGAGCCGTGAGGGAATTTATAAAGCTCTTTCCGGAACAGGAAACCCATCTTTTAAAACGATTTCAAAA
ATATCTAAAGCATTAGGTTTGAAAATACACTTCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I3XCB7

Antitoxin

• AlphaFold DB: A0A1I3XCJ1