Detailed information of TA system TA065841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2487866..2488403
• Replicon: chromosome
• Accession: NZ_CP017053
• Organism: Burkholderia pseudomallei strain MSHR3763

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BGI46_RS33945
• Protein ID: WP_009923450.1
• Coordinates: 2487866..2488135 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BGI46_RS33950
• Protein ID: WP_004525420.1
• Coordinates: 2488119..2488403 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGI46_RS41185	2483843..2484271	-	429	Protein_2048	DDE-type integrase/transposase/recombinase	-
BGI46_RS41190	2484269..2485042	-	774	Protein_2049	prohibitin family protein	-
BGI46_RS33910	2485039..2485188	-	150	WP_004542626.1	bacteriophage protein Gp44	-
BGI46_RS33915	2485444..2486025	+	582	WP_004542548.1	hypothetical protein	-
BGI46_RS41195	2486256..2486555	-	300	WP_160973881.1	hypothetical protein	-
BGI46_RS33925	2486522..2486743	-	222	WP_004525418.1	hypothetical protein	-
BGI46_RS33940	2487020..2487295	-	276	WP_004552388.1	bacteriophage protein Gp49	-
BGI46_RS33945	2487866..2488135	+	270	WP_009923450.1	BrnT family toxin	Toxin
BGI46_RS33950	2488119..2488403	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BGI46_RS33965	2489050..2489433	+	384	Protein_2057	DDE-type integrase/transposase/recombinase	-
BGI46_RS33970	2489396..2489824	+	429	Protein_2058	IS110 family transposase	-
BGI46_RS33975	2490051..2490167	-	117	Protein_2059	IS3 family transposase	-
BGI46_RS33980	2490414..2491874	+	1461	WP_020850157.1	metallophosphoesterase	-
BGI46_RS33985	2492033..2493295	+	1263	WP_020850375.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2481532..2491874	10342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T65841 WP_009923450.1 NZ_CP017053:2487866-2488135 [Burkholderia pseudomallei]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T65841 NZ_CP017053:2487866-2488135 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT65841 WP_004525420.1 NZ_CP017053:2488119-2488403 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT65841 NZ_CP017053:2488119-2488403 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8