Detailed information of TA system TA065838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3072226..3073267
• Replicon: chromosome
• Accession: NZ_CP017052
• Organism: Burkholderia pseudomallei strain MSHR3763

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BGI46_RS16265
• Protein ID: WP_004539802.1
• Coordinates: 3072692..3073267 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0E1SLI7
• Locus tag: BGI46_RS16260
• Protein ID: WP_004540053.1
• Coordinates: 3072226..3072687 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGI46_RS16225	3067832..3068500	-	669	WP_012730038.1	restriction endonuclease fold toxin 5 domain-containing protein	-
BGI46_RS16235	3068785..3069291	-	507	WP_004539924.1	DUF4123 domain-containing protein	-
BGI46_RS16240	3069288..3069713	-	426	WP_004539640.1	PAAR domain-containing protein	-
BGI46_RS39615	3070111..3070491	-	381	WP_020850431.1	hypothetical protein	-
BGI46_RS16250	3070951..3071178	-	228	WP_004540114.1	hypothetical protein	-
BGI46_RS39620	3071175..3071573	-	399	WP_133604798.1	hypothetical protein	-
BGI46_RS16260	3072226..3072687	+	462	WP_004540053.1	excisionase family DNA-binding protein	Antitoxin
BGI46_RS16265	3072692..3073267	+	576	WP_004539802.1	PIN domain-containing protein	Toxin
BGI46_RS16270	3073453..3075669	+	2217	WP_004540097.1	ATP-binding protein	-
BGI46_RS16290	3076918..3077097	+	180	WP_004191077.1	hypothetical protein	-
BGI46_RS16300	3077281..3077553	+	273	WP_004527347.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (83-129)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 22056.35 Da
• Isoelectric Point: 6.4079

>T65838 WP_004539802.1 NZ_CP017052:3072692-3073267 [Burkholderia pseudomallei]
MSSNFTVVYDACVLYPAPLRDLLMHLAVTDLYRARWTDMIHDEWTRNLLAKRTDLKPEAIQRTRELMDMHVRDAKVENFE
HLIAAIELPDPDDRHVVAAAMHAGAELIVTMNIKDFPRERVGKYNVSAQHPDDFIVDLWDLNKHKVMEAVARHRKSMKNP
PKSVEDYLDTMANQGLVQTVDALRPYAPWAI

Nucleotide

• Length: 576 bp

>T65838 NZ_CP017052:3072692-3073267 [Burkholderia pseudomallei]
ATGTCGTCGAACTTTACCGTTGTCTACGATGCGTGCGTCCTATATCCGGCTCCGTTGCGCGACTTGTTGATGCATCTCGC
GGTGACGGATCTGTACCGCGCGCGTTGGACCGATATGATTCATGACGAGTGGACAAGGAATTTGCTCGCGAAGCGAACCG
ACCTGAAACCCGAGGCGATCCAGCGCACGCGGGAACTGATGGACATGCATGTTCGCGACGCGAAAGTGGAAAACTTCGAG
CATCTGATTGCGGCGATCGAGTTGCCCGATCCTGATGATCGTCATGTCGTCGCGGCAGCCATGCATGCTGGGGCGGAACT
CATCGTCACGATGAACATCAAGGATTTCCCGAGGGAGCGGGTCGGAAAGTACAACGTGTCGGCTCAACATCCCGACGATT
TCATCGTCGACTTGTGGGACTTGAACAAGCACAAGGTGATGGAGGCGGTCGCGCGACATCGCAAGTCGATGAAGAACCCG
CCGAAGTCAGTGGAAGACTATCTCGACACGATGGCCAATCAGGGACTCGTGCAGACGGTCGATGCGCTGCGGCCGTATGC
GCCTTGGGCTATCTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17155.41 Da
• Isoelectric Point: 5.5474

>AT65838 WP_004540053.1 NZ_CP017052:3072226-3072687 [Burkholderia pseudomallei]
MNTTVTPAALPSEHEAELARESRRNLVTVVNSHAETQQFDFKDEKGETHTVTLPTSALRLLDEILHQIGNGNAVSIIPIH
AELTTQQAADLLNVSRPYLVQLLEQGEIPFRKVNTHRRVMYLDLIQYKNKIDANRLDALAELTAQAQELGMGY

Nucleotide

• Length: 462 bp

>AT65838 NZ_CP017052:3072226-3072687 [Burkholderia pseudomallei]
ATGAACACCACTGTCACTCCTGCGGCCCTCCCGTCCGAACACGAGGCCGAGCTCGCGCGCGAGTCGCGGCGGAATTTGGT
CACGGTCGTGAACAGCCACGCCGAGACTCAGCAGTTTGATTTCAAGGACGAAAAGGGGGAGACGCATACAGTCACGCTTC
CGACCTCGGCGTTAAGGCTGCTAGACGAGATCCTCCATCAGATCGGAAATGGCAACGCGGTGTCGATCATTCCAATCCAT
GCCGAATTGACGACCCAGCAAGCCGCCGACCTTCTGAATGTCTCGCGTCCGTACCTGGTTCAACTTCTTGAGCAGGGAGA
GATCCCATTCCGGAAGGTAAATACGCATCGACGGGTGATGTATCTGGATCTGATTCAGTACAAAAACAAGATCGATGCGA
ACCGTCTCGACGCGCTGGCTGAACTGACGGCTCAGGCGCAGGAACTTGGAATGGGATATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1SLI7