Detailed information of TA system TA065835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2487926..2488463
• Replicon: chromosome
• Accession: NZ_CP017051
• Organism: Burkholderia pseudomallei strain MSHR4083

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BGI47_RS33950
• Protein ID: WP_009923450.1
• Coordinates: 2487926..2488195 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BGI47_RS33955
• Protein ID: WP_004525420.1
• Coordinates: 2488179..2488463 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGI47_RS41075	2483903..2484331	-	429	Protein_2046	DDE-type integrase/transposase/recombinase	-
BGI47_RS41080	2484329..2485102	-	774	Protein_2047	prohibitin family protein	-
BGI47_RS33915	2485099..2485248	-	150	WP_004542626.1	bacteriophage protein Gp44	-
BGI47_RS33920	2485504..2486085	+	582	WP_004542548.1	hypothetical protein	-
BGI47_RS40550	2486316..2486534	-	219	WP_004552392.1	hypothetical protein	-
BGI47_RS33930	2486582..2486803	-	222	WP_004525418.1	hypothetical protein	-
BGI47_RS33945	2487080..2487355	-	276	WP_004552388.1	bacteriophage protein Gp49	-
BGI47_RS33950	2487926..2488195	+	270	WP_009923450.1	BrnT family toxin	Toxin
BGI47_RS33955	2488179..2488463	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BGI47_RS33970	2489110..2489493	+	384	Protein_2055	DDE-type integrase/transposase/recombinase	-
BGI47_RS33975	2489456..2489884	+	429	Protein_2056	IS110 family transposase	-
BGI47_RS33980	2490111..2490227	-	117	Protein_2057	IS3 family transposase	-
BGI47_RS33985	2490474..2491934	+	1461	WP_020850157.1	metallophosphoesterase	-
BGI47_RS33990	2492093..2493355	+	1263	WP_020850375.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2481592..2495577	13985

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T65835 WP_009923450.1 NZ_CP017051:2487926-2488195 [Burkholderia pseudomallei]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T65835 NZ_CP017051:2487926-2488195 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT65835 WP_004525420.1 NZ_CP017051:2488179-2488463 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT65835 NZ_CP017051:2488179-2488463 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8