65825

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2413246..2413783 Replicon chromosome
Accession NZ_CP017047
Organism Burkholderia pseudomallei strain MSHR6755

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BGI49_RS33595 Protein ID WP_004549731.1
Coordinates 2413246..2413515 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BGI49_RS33600 Protein ID WP_004525420.1
Coordinates 2413499..2413783 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGI49_RS40715 2408332..2409105 - 774 Protein_1974 prohibitin family protein -
BGI49_RS33550 2409102..2409251 - 150 WP_004537646.1 bacteriophage protein Gp44 -
BGI49_RS33555 2409308..2410528 - 1221 WP_004525642.1 ISL3-like element ISBma1 family transposase -
BGI49_RS33565 2410815..2411468 + 654 WP_038731741.1 hypothetical protein -
BGI49_RS40235 2411654..2411953 - 300 WP_160458000.1 hypothetical protein -
BGI49_RS33575 2411920..2412141 - 222 WP_029248167.1 hypothetical protein -
BGI49_RS33590 2412418..2412693 - 276 WP_038749212.1 hypothetical protein -
BGI49_RS33595 2413246..2413515 + 270 WP_004549731.1 BrnT family toxin Toxin
BGI49_RS33600 2413499..2413783 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BGI49_RS33615 2414429..2414812 + 384 Protein_1983 DDE-type integrase/transposase/recombinase -
BGI49_RS33620 2414775..2415203 + 429 Protein_1984 IS110 family transposase -
BGI49_RS33625 2415430..2415546 - 117 Protein_1985 IS3 family transposase -
BGI49_RS33630 2415812..2417272 + 1461 WP_076849498.1 metallophosphoesterase -
BGI49_RS33635 2417431..2418693 + 1263 WP_038765621.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 2405281..2420914 15633


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T65825 WP_004549731.1 NZ_CP017047:2413246-2413515 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T65825 NZ_CP017047:2413246-2413515 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAACATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT65825 WP_004525420.1 NZ_CP017047:2413499-2413783 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT65825 NZ_CP017047:2413499-2413783 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References