65821

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2434611..2435148 Replicon chromosome
Accession NZ_CP017045
Organism Burkholderia pseudomallei strain MSHR7929

Toxin (Protein)


Gene name brnT Uniprot ID A0A0F6L9W9
Locus tag BGI50_RS33450 Protein ID WP_009971435.1
Coordinates 2434611..2434880 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BGI50_RS33455 Protein ID WP_004525420.1
Coordinates 2434864..2435148 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGI50_RS40415 2430606..2431034 - 429 Protein_2015 DDE-type integrase/transposase/recombinase -
BGI50_RS40280 2431032..2431805 - 774 Protein_2016 prohibitin family protein -
BGI50_RS33415 2431802..2431951 - 150 WP_004537646.1 bacteriophage protein Gp44 -
BGI50_RS33420 2432207..2432788 + 582 WP_004542548.1 hypothetical protein -
BGI50_RS40285 2433019..2433318 - 300 WP_161788725.1 hypothetical protein -
BGI50_RS33430 2433285..2433506 - 222 WP_029248167.1 hypothetical protein -
BGI50_RS33445 2433783..2434058 - 276 WP_076847323.1 hypothetical protein -
BGI50_RS33450 2434611..2434880 + 270 WP_009971435.1 BrnT family toxin Toxin
BGI50_RS33455 2434864..2435148 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BGI50_RS33470 2435795..2436178 + 384 Protein_2024 DDE-type integrase/transposase/recombinase -
BGI50_RS33475 2436141..2436569 + 429 Protein_2025 IS110 family transposase -
BGI50_RS33480 2436796..2436912 - 117 Protein_2026 IS3 family transposase -
BGI50_RS33485 2437159..2438619 + 1461 WP_038726744.1 metallophosphoesterase -
BGI50_RS33490 2438778..2440040 + 1263 WP_004537187.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2367253..2442262 75009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10143.43 Da        Isoelectric Point: 5.6831

>T65821 WP_009971435.1 NZ_CP017045:2434611-2434880 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVVGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T65821 NZ_CP017045:2434611-2434880 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGGTCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT65821 WP_004525420.1 NZ_CP017045:2434864-2435148 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT65821 NZ_CP017045:2434864-2435148 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6L9W9

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References