Detailed information of TA system TA065820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2393218..2393755
• Replicon: chromosome
• Accession: NZ_CP017045
• Organism: Burkholderia pseudomallei strain MSHR7929

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: BGI50_RS33205
• Protein ID: WP_004549731.1
• Coordinates: 2393218..2393487 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BGI50_RS33210
• Protein ID: WP_004525420.1
• Coordinates: 2393471..2393755 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGI50_RS33155	2388263..2389084	+	822	WP_076847305.1	hypothetical protein	-
BGI50_RS33160	2389081..2389341	+	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
BGI50_RS33165	2389495..2390487	+	993	WP_015967393.1	hypothetical protein	-
BGI50_RS33170	2390484..2391020	+	537	WP_024429363.1	DUF1064 domain-containing protein	-
BGI50_RS33175	2391030..2391374	+	345	WP_143297881.1	winged helix-turn-helix domain-containing protein	-
BGI50_RS33180	2391371..2391718	+	348	WP_043289604.1	DUF2591 family protein	-
BGI50_RS33185	2391733..2392131	+	399	WP_038738296.1	DUF1364 family protein	-
BGI50_RS33190	2392161..2392493	+	333	WP_038725178.1	hypothetical protein	-
BGI50_RS33195	2392469..2392729	+	261	WP_038725177.1	hypothetical protein	-
BGI50_RS33200	2392726..2393169	+	444	WP_004548453.1	hypothetical protein	-
BGI50_RS33205	2393218..2393487	+	270	WP_004549731.1	BrnT family toxin	Toxin
BGI50_RS33210	2393471..2393755	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BGI50_RS33215	2394017..2394928	+	912	WP_143281104.1	hypothetical protein	-
BGI50_RS33220	2394930..2395508	+	579	WP_038738294.1	hypothetical protein	-
BGI50_RS33225	2395597..2395953	+	357	WP_024429281.1	HNH endonuclease	-
BGI50_RS33230	2396087..2396572	+	486	WP_015967347.1	phage terminase small subunit P27 family	-
BGI50_RS33235	2396582..2398297	+	1716	WP_076847306.1	terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2367253..2442262	75009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T65820 WP_004549731.1 NZ_CP017045:2393218-2393487 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T65820 NZ_CP017045:2393218-2393487 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT65820 WP_004525420.1 NZ_CP017045:2393471-2393755 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT65820 NZ_CP017045:2393471-2393755 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8