Detailed information of TA system TA065818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1710556..1711211
• Replicon: chromosome
• Accession: NZ_CP017044
• Organism: Burkholderia pseudomallei strain MSHR7929

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BGI50_RS08990
• Protein ID: WP_173671360.1
• Coordinates: 1710915..1711211 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BGI50_RS08985
• Protein ID: WP_015985014.1
• Coordinates: 1710556..1710912 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGI50_RS08965	1705691..1706704	-	1014	WP_004531048.1	phage major capsid protein, P2 family	-
BGI50_RS08970	1706738..1707547	-	810	WP_004552664.1	GPO family capsid scaffolding protein	-
BGI50_RS08975	1707691..1709460	+	1770	WP_043289553.1	terminase ATPase subunit family protein	-
BGI50_RS08980	1709457..1710512	+	1056	WP_038724553.1	phage portal protein	-
BGI50_RS08985	1710556..1710912	-	357	WP_015985014.1	putative addiction module antidote protein	Antitoxin
BGI50_RS08990	1710915..1711211	-	297	WP_173671360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BGI50_RS08995	1712234..1713028	+	795	WP_052139734.1	NYN domain-containing protein	-
BGI50_RS09000	1713173..1713382	-	210	WP_076846667.1	hypothetical protein	-
BGI50_RS09005	1713776..1714339	-	564	WP_080312949.1	ImmA/IrrE family metallo-endopeptidase	-
BGI50_RS09010	1714348..1714701	-	354	WP_038740386.1	helix-turn-helix domain-containing protein	-
BGI50_RS09015	1715197..1715709	-	513	WP_076846668.1	helix-turn-helix domain-containing protein	-
BGI50_RS09020	1715834..1716013	-	180	WP_076804979.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1676998..1727718	50720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10761.57 Da
• Isoelectric Point: 9.9462

>T65818 WP_173671360.1 NZ_CP017044:c1711211-1710915 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQCRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T65818 NZ_CP017044:c1711211-1710915 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTCGACGGGCTTCGCGACCCGGTCGGTAGCGCGGCGATCAA
CCTGCGCATCGAGCGGGCAAAGCTTGGCAATCTCGGCCAATGTCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTATTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12575.37 Da
• Isoelectric Point: 9.9470

>AT65818 WP_015985014.1 NZ_CP017044:c1710912-1710556 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAESAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT65818 NZ_CP017044:c1710912-1710556 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGGAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAATCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA