Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 27533..28074 Replicon plasmid pMSCT44kb
Accession NZ_CP017022
Organism Xanthomonas citri pv. malvacearum strain MSCT

Toxin (Protein)


Gene name mazF Uniprot ID A0A220WPB1
Locus tag BGK55_RS21725 Protein ID WP_005917856.1
Coordinates 27751..28074 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A220WPJ3
Locus tag BGK55_RS23855 Protein ID WP_005917854.1
Coordinates 27533..27754 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGK55_RS21680 22662..22976 - 315 WP_005918227.1 hypothetical protein -
BGK55_RS21685 23121..23444 - 324 WP_005918229.1 hypothetical protein -
BGK55_RS21690 23441..24067 - 627 WP_005918230.1 ParA family protein -
BGK55_RS25310 24236..24373 + 138 WP_005918231.1 hypothetical protein -
BGK55_RS21695 24786..25163 - 378 Protein_21 hypothetical protein -
BGK55_RS21710 26402..26665 - 264 WP_005917846.1 hypothetical protein -
BGK55_RS21715 26690..27091 - 402 WP_005917849.1 type II toxin-antitoxin system VapC family toxin -
BGK55_RS21720 27088..27348 - 261 WP_005917852.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BGK55_RS23855 27533..27754 + 222 WP_005917854.1 antitoxin MazE family protein Antitoxin
BGK55_RS21725 27751..28074 + 324 WP_005917856.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BGK55_RS21730 28100..28387 + 288 WP_029220894.1 hypothetical protein -
BGK55_RS21735 28394..29581 + 1188 WP_005917859.1 zeta toxin family protein -
BGK55_RS21740 29767..30051 - 285 WP_003490829.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..43945 43945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(9-72)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11513.56 Da        Isoelectric Point: 6.2913

>T65816 WP_005917856.1 NZ_CP017022:27751-28074 [Xanthomonas citri pv. malvacearum]
MTQRGDLVTVALPGAYGKPRPALVIQSDLLAELESVLVCPITSDLRDAMFRVTVEPSLTNGLQKISQVMVDKTAPIPRAK
VSEPIGTLDAERMRAVERALMLVTGLA

Download         Length: 324 bp

>T65816 NZ_CP017022:27751-28074 [Xanthomonas citri pv. malvacearum]
ATGACGCAGCGTGGCGACTTGGTAACGGTGGCTTTGCCGGGAGCCTACGGCAAGCCACGGCCGGCGCTAGTCATCCAGTC
CGATTTGCTTGCCGAGCTGGAAAGCGTCCTCGTATGCCCGATCACAAGCGATCTGCGCGACGCGATGTTTCGGGTCACGG
TCGAGCCGAGTCTGACCAATGGTCTACAGAAGATTTCGCAGGTCATGGTGGACAAGACAGCACCGATACCTCGCGCGAAA
GTCAGTGAGCCGATTGGCACGCTCGACGCAGAACGCATGAGGGCCGTCGAACGCGCCCTCATGCTCGTCACCGGGCTGGC
ATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8240.23 Da        Isoelectric Point: 8.1023

>AT65816 WP_005917854.1 NZ_CP017022:27533-27754 [Xanthomonas citri pv. malvacearum]
MPRTHNPTNKVTRHRDRLRAAGYRPVQFWVHDTRSPELAAMLSRQCRSLHGDPTETDAIGFGEAAAGLIEGWE

Download         Length: 222 bp

>AT65816 NZ_CP017022:27533-27754 [Xanthomonas citri pv. malvacearum]
ATGCCGAGAACTCACAATCCGACGAACAAGGTCACCCGTCATCGTGACCGACTGCGTGCAGCCGGGTACAGGCCAGTGCA
GTTTTGGGTGCACGATACCCGCTCGCCCGAGTTGGCTGCGATGCTCAGCCGCCAGTGTCGCAGCCTGCATGGTGATCCAA
CCGAAACCGATGCTATCGGCTTCGGTGAAGCCGCCGCTGGCCTTATCGAGGGGTGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A220WPB1

Antitoxin

Source ID Structure
AlphaFold DB A0A220WPJ3

References