Detailed information of TA system TA065811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2623120..2623726
• Replicon: chromosome
• Accession: NZ_CP017019
• Organism: Moorella thermoacetica strain DSM 103132

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1D7XDY8
• Locus tag: Maut_RS13240
• Protein ID: WP_011393652.1
• Coordinates: 2623120..2623470 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Maut_RS13245
• Protein ID: WP_036371414.1
• Coordinates: 2623472..2623726 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Maut_RS13205	2618727..2619212	-	486	WP_036371410.1	ribosomal protein S18-alanine N-acetyltransferase	-
Maut_RS13210	2619230..2619916	-	687	WP_011393646.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
Maut_RS13215	2619897..2620364	-	468	WP_069591080.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
Maut_RS13220	2620525..2621100	-	576	WP_069591534.1	uracil-DNA glycosylase	-
Maut_RS13225	2621155..2621787	-	633	WP_069591535.1	thiamine phosphate synthase	-
Maut_RS13230	2621868..2622257	-	390	WP_025773384.1	response regulator	-
Maut_RS13235	2622405..2623106	-	702	WP_069591083.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
Maut_RS13240	2623120..2623470	-	351	WP_011393652.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Maut_RS13245	2623472..2623726	-	255	WP_036371414.1	ribbon-helix-helix protein, CopG family	Antitoxin
Maut_RS13250	2623854..2624975	-	1122	WP_069591085.1	alanine racemase	-
Maut_RS13255	2624947..2626539	-	1593	WP_069591087.1	NAD(P)H-hydrate dehydratase	-
Maut_RS13260	2626544..2626930	-	387	WP_081328620.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12684.71 Da
• Isoelectric Point: 7.2034

>T65811 WP_011393652.1 NZ_CP017019:c2623470-2623120 [Moorella thermoacetica]
MLVRRGDVFYAHLNPVVGSEQGGTRPVLIIQNDIGNQYSPTTIVAAITSQIAKAKLPTHVEISAAKSGLERDSVILLEQI
RTIDKSRLKQKVAVLDDEILEKVNRALEISLGLVEV

Nucleotide

• Length: 351 bp

>T65811 NZ_CP017019:c2623470-2623120 [Moorella thermoacetica]
ATGCTGGTACGGCGCGGGGATGTCTTTTACGCCCATCTCAATCCGGTGGTCGGTTCCGAGCAAGGCGGAACCCGCCCGGT
ACTTATCATTCAAAATGACATTGGCAACCAGTATAGCCCCACTACTATTGTGGCCGCTATTACTTCCCAGATTGCCAAGG
CCAAACTGCCCACCCATGTGGAAATCAGCGCTGCCAAGAGCGGCCTGGAGCGCGATTCTGTTATCCTGCTGGAACAGATC
CGCACCATAGACAAGAGCCGCCTCAAACAAAAGGTGGCCGTCCTGGATGATGAGATCCTGGAAAAGGTGAACCGGGCCCT
GGAAATAAGCCTGGGACTGGTGGAAGTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9983.45 Da
• Isoelectric Point: 6.3653

>AT65811 WP_036371414.1 NZ_CP017019:c2623726-2623472 [Moorella thermoacetica]
MISLPESLLAEVDGLANLEKRNRSEFIREAMKLYITERKRRNIREQMKRGYQEMAPINLALAVENYDVENEVQRYYDKSL
AECK

Nucleotide

• Length: 255 bp

>AT65811 NZ_CP017019:c2623726-2623472 [Moorella thermoacetica]
ATGATCAGCTTGCCGGAGAGCCTCCTGGCCGAGGTTGACGGCCTGGCGAACCTTGAAAAGCGTAACCGCAGCGAGTTTAT
CCGGGAAGCTATGAAACTATATATTACCGAGCGTAAAAGGCGCAATATCCGGGAACAAATGAAACGGGGATACCAGGAAA
TGGCACCTATCAATTTAGCCCTTGCAGTGGAAAACTATGATGTAGAGAATGAAGTACAAAGATACTACGATAAAAGCCTG
GCGGAGTGTAAATAA

Structures

Toxin

• AlphaFold DB: A0A1D7XDY8