Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 243003..243645 Replicon chromosome
Accession NZ_CP017016
Organism Bacillus sp. ABP14

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BGI23_RS01370 Protein ID WP_000635963.1
Coordinates 243295..243645 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BGI23_RS01365 Protein ID WP_000004570.1
Coordinates 243003..243290 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGI23_RS01340 238328..239290 + 963 WP_070805101.1 UV DNA damage repair endonuclease UvsE -
BGI23_RS01345 239283..239855 - 573 WP_000906920.1 rhomboid family intramembrane serine protease -
BGI23_RS01350 239948..240307 + 360 WP_000635037.1 holo-ACP synthase -
BGI23_RS01355 240464..241414 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BGI23_RS01360 241532..242701 + 1170 WP_000390609.1 alanine racemase -
BGI23_RS01365 243003..243290 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BGI23_RS01370 243295..243645 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BGI23_RS01375 243713..245881 + 2169 WP_070805102.1 RNA-binding transcriptional accessory protein -
BGI23_RS01380 245939..246055 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BGI23_RS01385 246251..246709 + 459 WP_000344246.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T65808 WP_000635963.1 NZ_CP017016:243295-243645 [Bacillus sp. ABP14]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T65808 NZ_CP017016:243295-243645 [Bacillus sp. ABP14]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT65808 WP_000004570.1 NZ_CP017016:243003-243290 [Bacillus sp. ABP14]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT65808 NZ_CP017016:243003-243290 [Bacillus sp. ABP14]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References