Detailed information of TA system TA065805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 15222..15814
• Replicon: plasmid pPsa22180b
• Accession: NZ_CP017011
• Organism: Pseudomonas syringae pv. actinidiae strain NZ-47

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A2P0QFS1
• Locus tag: PsaNZ47_RS32130
• Protein ID: WP_074321559.1
• Coordinates: 15222..15524 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A2P0QFH5
• Locus tag: PsaNZ47_RS32135
• Protein ID: WP_074321561.1
• Coordinates: 15521..15814 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsaNZ47_RS32095	10355..10807	-	453	WP_074321593.1	hypothetical protein	-
PsaNZ47_RS32100	10953..11264	-	312	WP_074321591.1	hypothetical protein	-
PsaNZ47_RS32105	11348..11779	-	432	WP_074321589.1	hypothetical protein	-
PsaNZ47_RS32110	12715..13011	-	297	WP_074321550.1	hypothetical protein	-
PsaNZ47_RS32115	13146..13583	-	438	WP_074321552.1	hypothetical protein	-
PsaNZ47_RS32120	13641..14477	-	837	WP_074321554.1	DUF945 domain-containing protein	-
PsaNZ47_RS32125	14666..15103	-	438	WP_139301616.1	hypothetical protein	-
PsaNZ47_RS32130	15222..15524	+	303	WP_074321559.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PsaNZ47_RS32135	15521..15814	+	294	WP_074321561.1	putative addiction module antidote protein	Antitoxin
PsaNZ47_RS32145	16826..17131	-	306	WP_074321288.1	hypothetical protein	-
PsaNZ47_RS32155	17612..17851	-	240	WP_019333459.1	hypothetical protein	-
PsaNZ47_RS32160	18160..18339	-	180	Protein_21	hypothetical protein	-
PsaNZ47_RS32165	18405..18692	-	288	WP_074321290.1	hypothetical protein	-
PsaNZ47_RS32175	18962..19168	-	207	WP_074321348.1	hypothetical protein	-
PsaNZ47_RS32180	19336..19626	-	291	WP_074321292.1	hypothetical protein	-
PsaNZ47_RS32185	19623..20057	-	435	WP_074321293.1	hypothetical protein	-
PsaNZ47_RS32190	20314..20523	-	210	WP_074321294.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55944	55944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-91)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11134.67 Da
• Isoelectric Point: 9.9232

>T65805 WP_074321559.1 NZ_CP017011:15222-15524 [Pseudomonas syringae pv. actinidiae]
MKTIKQTATFRTWESKLKDNRAKAAIAARILRVANGLMGDVSPVGQGVSELRIHYGPGYRVYFQQRGNELVILLCGGDKS
SQARDIETAKTLAHDWSENE

Nucleotide

• Length: 303 bp

>T65805 NZ_CP017011:15222-15524 [Pseudomonas syringae pv. actinidiae]
ATGAAAACGATCAAACAAACCGCAACGTTCCGCACCTGGGAAAGCAAACTCAAGGACAACCGAGCCAAGGCCGCTATTGC
AGCTCGTATCTTGCGCGTAGCCAACGGCTTGATGGGAGACGTTTCACCTGTCGGCCAAGGGGTCAGCGAGTTACGCATTC
ACTATGGCCCGGGTTATAGGGTCTACTTCCAACAACGGGGTAACGAGCTGGTGATCCTGCTGTGCGGGGGGGACAAATCC
TCTCAGGCTCGCGACATAGAGACTGCCAAAACACTGGCTCATGACTGGAGTGAAAATGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 5.0197

>AT65805 WP_074321561.1 NZ_CP017011:15521-15814 [Pseudomonas syringae pv. actinidiae]
MKESLYDYDPAAALVDAESITVFIADAFETGDAAHIAQALGVVARAKGMSEIAKVSGLSRESLYVAFSDKGNPTLKTMLA
VMRAVGVDMTARAHSPT

Nucleotide

• Length: 294 bp

>AT65805 NZ_CP017011:15521-15814 [Pseudomonas syringae pv. actinidiae]
ATGAAAGAATCACTGTATGACTACGACCCAGCCGCGGCGCTGGTCGACGCTGAAAGCATCACAGTCTTCATTGCTGATGC
GTTTGAAACGGGCGATGCCGCACATATCGCACAGGCCCTTGGTGTGGTGGCACGCGCCAAGGGTATGAGCGAGATAGCGA
AGGTATCAGGGCTATCACGCGAATCGCTTTACGTGGCGTTTAGTGACAAGGGTAATCCGACGCTGAAAACCATGCTTGCC
GTGATGCGCGCCGTAGGGGTAGACATGACAGCACGTGCCCATTCACCGACTTAA

Structures

Toxin

• AlphaFold DB: A0A2P0QFS1

Antitoxin

• AlphaFold DB: A0A2P0QFH5