Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/Xre-HipA |
| Location | 723011..724609 | Replicon | chromosome |
| Accession | NC_004347 | ||
| Organism | Shewanella oneidensis MR-1 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | Q8EIX3 |
| Locus tag | SO_0706 | Protein ID | WP_011071024.1 |
| Coordinates | 723308..724609 (+) | Length | 434 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | Q8EIX4 |
| Locus tag | SO_0705 | Protein ID | WP_011071023.1 |
| Coordinates | 723068..723304 (+) | Length | 79 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SO_RS03320 (SO_0701) | 718091..719080 | + | 990 | WP_011071019.1 | LysR family transcriptional regulator | - |
| SO_RS03325 (SO_0702) | 719084..720442 | - | 1359 | WP_011071020.1 | MATE family efflux transporter | - |
| SO_RS03330 (SO_0703) | 720670..720960 | + | 291 | WP_011071021.1 | co-chaperone GroES | - |
| SO_RS03335 (SO_0704) | 721019..722656 | + | 1638 | WP_011071022.1 | chaperonin GroEL | - |
| SO_RS03340 (SO_0705) | 723068..723304 | + | 237 | WP_011071023.1 | type II toxin-antitoxin system antitoxin HipB | Antitoxin |
| SO_RS03345 (SO_0706) | 723308..724609 | + | 1302 | WP_011071024.1 | type II toxin-antitoxin system toxin serine/threonine-protein kinase HipA | Toxin |
| SO_RS03350 | 725102..725668 | - | 567 | WP_164925615.1 | hypothetical protein | - |
| SO_RS03355 (SO_0708) | 725665..726876 | - | 1212 | WP_011071025.1 | IS256-like element ISSod18 family transposase | - |
| SO_RS03360 | 726928..727845 | - | 918 | WP_164925616.1 | hypothetical protein | - |
| SO_RS03365 (SO_0710) | 727827..729254 | - | 1428 | WP_164925617.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 725665..726876 | 1211 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 434 a.a. Molecular weight: 48732.05 Da Isoelectric Point: 5.4777
>T658 WP_011071024.1 NC_004347:723308-724609 [Shewanella oneidensis MR-1]
MSTAKTLTLEMHLGDLMIGELSFDATADTFAVHYTKDWQQSGFPLSPTIPLDGTGTSNQISMFLVNLLPENKGLDYLIES
LGVSKGNTFALIRAIGLDTAGAIAFVPKGALLPETQLRPIKAEEVIQRIEDPTMWPMEIWDGKPRLSVAGVQPKLNLFYN
GKEFAFAEGTLSSTHIVKFEKYHHLVINEFITMRLAKVLGMNVANVDIVHFGRYKALCVERFDRRNIPGEQRVLRRHIVD
SCQALGFSVSKKYERNFGTGRDVKDIREGVSFNRLFSLAAKCRNPVAAKQDMLQWALFNLLTGNADAHGKNYSFFMTPSG
MEPTPWYDLVSVDMYEDFEQQLAMAIDDEFDPNSIYAYQLAAFMDGLGLPRNLLISNLTRIARRIPQAIAEVILMLPPLD
EDEASFVAHYKTQLLARCERYLGFVDEVRDVEV
MSTAKTLTLEMHLGDLMIGELSFDATADTFAVHYTKDWQQSGFPLSPTIPLDGTGTSNQISMFLVNLLPENKGLDYLIES
LGVSKGNTFALIRAIGLDTAGAIAFVPKGALLPETQLRPIKAEEVIQRIEDPTMWPMEIWDGKPRLSVAGVQPKLNLFYN
GKEFAFAEGTLSSTHIVKFEKYHHLVINEFITMRLAKVLGMNVANVDIVHFGRYKALCVERFDRRNIPGEQRVLRRHIVD
SCQALGFSVSKKYERNFGTGRDVKDIREGVSFNRLFSLAAKCRNPVAAKQDMLQWALFNLLTGNADAHGKNYSFFMTPSG
MEPTPWYDLVSVDMYEDFEQQLAMAIDDEFDPNSIYAYQLAAFMDGLGLPRNLLISNLTRIARRIPQAIAEVILMLPPLD
EDEASFVAHYKTQLLARCERYLGFVDEVRDVEV
Download Length: 1302 bp
>T658 NC_004347:723308-724609 [Shewanella oneidensis MR-1]
ATGAGTACAGCTAAAACGCTTACGCTAGAGATGCACCTCGGTGATTTAATGATCGGGGAGTTATCCTTTGATGCCACGGC
CGACACCTTTGCCGTGCATTACACCAAAGACTGGCAGCAAAGTGGATTTCCACTTTCACCAACTATTCCCTTAGATGGCA
CAGGAACGAGCAATCAAATATCAATGTTTTTGGTTAACTTATTGCCAGAAAACAAAGGATTAGATTATCTGATTGAATCG
CTTGGCGTCTCTAAGGGGAATACCTTTGCCTTGATCCGCGCCATTGGTCTCGATACCGCAGGCGCCATTGCTTTCGTTCC
TAAAGGGGCTTTACTGCCTGAAACACAATTGCGGCCAATTAAGGCTGAAGAAGTTATTCAACGCATTGAAGATCCCACAA
TGTGGCCGATGGAAATTTGGGACGGCAAACCTCGACTATCGGTAGCGGGTGTTCAACCTAAATTAAACCTGTTTTATAAC
GGGAAAGAATTTGCCTTTGCCGAAGGCACGCTGTCATCGACCCATATTGTTAAATTCGAAAAATATCATCATTTAGTGAT
CAATGAATTTATAACGATGCGCTTAGCCAAAGTGCTTGGGATGAATGTGGCGAATGTCGATATTGTTCATTTTGGCCGTT
ACAAAGCCCTTTGTGTTGAACGCTTTGACAGACGCAATATTCCAGGCGAGCAAAGAGTGTTAAGAAGGCACATTGTTGAT
AGCTGCCAAGCACTTGGTTTTAGTGTAAGCAAAAAATATGAACGTAATTTTGGTACGGGGCGCGATGTTAAAGATATTCG
TGAGGGGGTAAGTTTTAACAGGCTATTTAGTCTTGCCGCAAAATGCCGCAATCCCGTGGCAGCTAAGCAGGATATGCTGC
AATGGGCGCTATTCAATTTACTGACCGGCAATGCCGATGCACACGGTAAAAACTACTCATTTTTTATGACGCCAAGCGGA
ATGGAGCCAACTCCTTGGTACGATTTGGTCAGCGTAGATATGTATGAGGATTTTGAACAACAGCTTGCGATGGCTATTGA
TGACGAGTTTGATCCAAATAGCATTTACGCATATCAATTAGCCGCATTTATGGATGGTTTGGGTTTACCAAGAAATTTAT
TAATTAGTAACTTAACGAGAATAGCGCGAAGAATACCGCAGGCAATAGCGGAGGTGATTTTAATGCTCCCGCCCCTTGAT
GAAGATGAAGCATCATTTGTGGCTCATTACAAAACCCAGTTACTGGCGAGATGTGAACGTTACCTTGGTTTTGTTGACGA
GGTCAGGGATGTGGAAGTGTAG
ATGAGTACAGCTAAAACGCTTACGCTAGAGATGCACCTCGGTGATTTAATGATCGGGGAGTTATCCTTTGATGCCACGGC
CGACACCTTTGCCGTGCATTACACCAAAGACTGGCAGCAAAGTGGATTTCCACTTTCACCAACTATTCCCTTAGATGGCA
CAGGAACGAGCAATCAAATATCAATGTTTTTGGTTAACTTATTGCCAGAAAACAAAGGATTAGATTATCTGATTGAATCG
CTTGGCGTCTCTAAGGGGAATACCTTTGCCTTGATCCGCGCCATTGGTCTCGATACCGCAGGCGCCATTGCTTTCGTTCC
TAAAGGGGCTTTACTGCCTGAAACACAATTGCGGCCAATTAAGGCTGAAGAAGTTATTCAACGCATTGAAGATCCCACAA
TGTGGCCGATGGAAATTTGGGACGGCAAACCTCGACTATCGGTAGCGGGTGTTCAACCTAAATTAAACCTGTTTTATAAC
GGGAAAGAATTTGCCTTTGCCGAAGGCACGCTGTCATCGACCCATATTGTTAAATTCGAAAAATATCATCATTTAGTGAT
CAATGAATTTATAACGATGCGCTTAGCCAAAGTGCTTGGGATGAATGTGGCGAATGTCGATATTGTTCATTTTGGCCGTT
ACAAAGCCCTTTGTGTTGAACGCTTTGACAGACGCAATATTCCAGGCGAGCAAAGAGTGTTAAGAAGGCACATTGTTGAT
AGCTGCCAAGCACTTGGTTTTAGTGTAAGCAAAAAATATGAACGTAATTTTGGTACGGGGCGCGATGTTAAAGATATTCG
TGAGGGGGTAAGTTTTAACAGGCTATTTAGTCTTGCCGCAAAATGCCGCAATCCCGTGGCAGCTAAGCAGGATATGCTGC
AATGGGCGCTATTCAATTTACTGACCGGCAATGCCGATGCACACGGTAAAAACTACTCATTTTTTATGACGCCAAGCGGA
ATGGAGCCAACTCCTTGGTACGATTTGGTCAGCGTAGATATGTATGAGGATTTTGAACAACAGCTTGCGATGGCTATTGA
TGACGAGTTTGATCCAAATAGCATTTACGCATATCAATTAGCCGCATTTATGGATGGTTTGGGTTTACCAAGAAATTTAT
TAATTAGTAACTTAACGAGAATAGCGCGAAGAATACCGCAGGCAATAGCGGAGGTGATTTTAATGCTCCCGCCCCTTGAT
GAAGATGAAGCATCATTTGTGGCTCATTACAAAACCCAGTTACTGGCGAGATGTGAACGTTACCTTGGTTTTGTTGACGA
GGTCAGGGATGTGGAAGTGTAG
Antitoxin
Download Length: 79 a.a. Molecular weight: 8501.82 Da Isoelectric Point: 6.2232
>AT658 WP_011071023.1 NC_004347:723068-723304 [Shewanella oneidensis MR-1]
MASPLNQQSLGLLIKERRKSAALTQDVAAMLCGVTKKTLIRVEKGEDVYISTVFKILDGLGIDIVSAQTSDTETNGWY
MASPLNQQSLGLLIKERRKSAALTQDVAAMLCGVTKKTLIRVEKGEDVYISTVFKILDGLGIDIVSAQTSDTETNGWY
Download Length: 237 bp
>AT658 NC_004347:723068-723304 [Shewanella oneidensis MR-1]
ATGGCATCACCGTTAAATCAACAATCCCTCGGCTTGTTAATTAAAGAACGCAGGAAAAGTGCTGCTCTTACTCAAGACGT
GGCGGCCATGCTGTGCGGCGTAACCAAAAAAACGCTGATCCGAGTTGAAAAGGGCGAGGATGTTTATATCTCAACCGTAT
TCAAAATCCTTGATGGTCTGGGGATTGATATCGTTTCGGCTCAAACCAGCGACACCGAAACTAACGGCTGGTATTAA
ATGGCATCACCGTTAAATCAACAATCCCTCGGCTTGTTAATTAAAGAACGCAGGAAAAGTGCTGCTCTTACTCAAGACGT
GGCGGCCATGCTGTGCGGCGTAACCAAAAAAACGCTGATCCGAGTTGAAAAGGGCGAGGATGTTTATATCTCAACCGTAT
TCAAAATCCTTGATGGTCTGGGGATTGATATCGTTTCGGCTCAAACCAGCGACACCGAAACTAACGGCTGGTATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10106 | Xenorhabdus nematophila ATCC 19061 |
34.286 |
89.744 |
0.308 |
Multiple sequence alignment
References
(1) Yurong Wen et al. (2014) The bacterial antitoxin HipB establishes a ternary complex with operator DNA and phosphorylated toxin HipA to regulate bacterial persistence. Nucleic Acids Research 42(15):10134-47. [PubMed:25056321]