Detailed information of TA system TA065796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE-Phd
• Location: 231..845
• Replicon: plasmid pPsa20586
• Accession: NZ_CP017008
• Organism: Pseudomonas syringae pv. actinidiae strain ICMP 20586

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A2V0R8Y7
• Locus tag: PsaNZ45_RS32175
• Protein ID: WP_017685123.1
• Coordinates: 495..845 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: S6SUK0
• Locus tag: PsaNZ45_RS32170
• Protein ID: WP_017685122.1
• Coordinates: 231..479 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsaNZ45_RS32170	231..479	+	249	WP_017685122.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PsaNZ45_RS32175	495..845	+	351	WP_017685123.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PsaNZ45_RS32180	977..1561	-	585	Protein_2	helix-turn-helix transcriptional regulator	-
PsaNZ45_RS32195	3268..3573	+	306	WP_017706770.1	type IV secretion system protein	-
PsaNZ45_RS32200	3563..4372	+	810	WP_017684942.1	P-type conjugative transfer protein VirB9	-
PsaNZ45_RS32205	4359..5729	+	1371	WP_017684943.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76088	76088

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13012.89 Da
• Isoelectric Point: 4.1953

>T65796 WP_017685123.1 NZ_CP017008:495-845 [Pseudomonas syringae pv. actinidiae]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI

Nucleotide

• Length: 351 bp

>T65796 NZ_CP017008:495-845 [Pseudomonas syringae pv. actinidiae]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9389.72 Da
• Isoelectric Point: 9.5019

>AT65796 WP_017685122.1 NZ_CP017008:231-479 [Pseudomonas syringae pv. actinidiae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK

Nucleotide

• Length: 249 bp

>AT65796 NZ_CP017008:231-479 [Pseudomonas syringae pv. actinidiae]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA

Structures

Toxin

• AlphaFold DB: A0A2V0R8Y7

Antitoxin

• AlphaFold DB: A0A2V0R8I6