Detailed information of TA system TA065794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5282402..5283036
• Replicon: chromosome
• Accession: NZ_CP017007
• Organism: Pseudomonas syringae pv. actinidiae strain ICMP 20586

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PsaNZ45_RS25685
• Protein ID: WP_003380365.1
• Coordinates: 5282632..5283036 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q888H8
• Locus tag: PsaNZ45_RS25680
• Protein ID: WP_003380366.1
• Coordinates: 5282402..5282632 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsaNZ45_RS25655	5277466..5278505	+	1040	Protein_4674	TRAP transporter substrate-binding protein	-
PsaNZ45_RS25660	5278569..5279096	+	528	WP_003380373.1	TRAP transporter small permease	-
PsaNZ45_RS25665	5279097..5280377	+	1281	WP_003380372.1	TRAP transporter large permease	-
PsaNZ45_RS25670	5280461..5281431	+	971	Protein_4677	DMT family transporter	-
PsaNZ45_RS25675	5281406..5282287	+	882	WP_003380368.1	aldose 1-epimerase	-
PsaNZ45_RS25680	5282402..5282632	+	231	WP_003380366.1	antitoxin	Antitoxin
PsaNZ45_RS25685	5282632..5283036	+	405	WP_003380365.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PsaNZ45_RS25690	5283223..5283798	-	576	WP_019334082.1	hypothetical protein	-
PsaNZ45_RS25695	5283797..5283985	+	189	WP_003380363.1	hypothetical protein	-
PsaNZ45_RS25700	5284221..5284733	+	513	WP_003380362.1	sigma-70 family RNA polymerase sigma factor	-
PsaNZ45_RS25705	5284733..5285395	+	663	WP_003380361.1	ChrR family anti-sigma-E factor	-
PsaNZ45_RS25710	5285471..5285707	-	237	WP_003380360.1	hypothetical protein	-
PsaNZ45_RS25715	5285772..5287028	-	1257	WP_003380359.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
PsaNZ45_RS25720	5287124..5287606	-	483	WP_003380358.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-34)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14814.09 Da
• Isoelectric Point: 6.8615

>T65794 WP_003380365.1 NZ_CP017007:5282632-5283036 [Pseudomonas syringae pv. actinidiae]
MLKYMLDTNICIFTIKNKPVSVREAFNLHHGQLCISAITLMELVYGAEKSSSPERNLAVVEGFAARLELLPYDSAAAAHT
GMIRAELARAGTPIGPYDQMIAGHARSLGLVVITNNQREFQRVEGLRVEDWVSQ

Nucleotide

• Length: 405 bp

>T65794 NZ_CP017007:5282632-5283036 [Pseudomonas syringae pv. actinidiae]
ATGCTCAAATACATGCTCGACACCAATATTTGCATCTTCACTATCAAGAACAAGCCGGTGTCGGTACGTGAGGCCTTCAA
CCTTCATCACGGGCAGCTGTGTATCAGCGCGATCACGTTGATGGAGCTTGTTTACGGCGCAGAAAAGTCTTCCAGTCCCG
AAAGAAATCTTGCTGTTGTAGAAGGTTTTGCAGCGCGTCTTGAATTGCTTCCCTACGATAGCGCCGCCGCGGCTCACACC
GGCATGATCAGAGCCGAGCTGGCCAGAGCGGGTACACCGATTGGCCCGTACGATCAAATGATTGCAGGACATGCCCGCTC
TCTTGGCCTCGTGGTCATCACCAACAACCAGCGTGAATTCCAGCGCGTCGAGGGGCTGAGGGTAGAAGACTGGGTAAGCC
AATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8593.67 Da
• Isoelectric Point: 4.5557

>AT65794 WP_003380366.1 NZ_CP017007:5282402-5282632 [Pseudomonas syringae pv. actinidiae]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPVEQLRESL

Nucleotide

• Length: 231 bp

>AT65794 NZ_CP017007:5282402-5282632 [Pseudomonas syringae pv. actinidiae]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGTCAGGCTGTTCGACTCCCAAAAGCGGTGGCATTACCCGACGACGT
GAAACGGGTAGACGTCGTAGCAGTGGGCAGGACACGCATCATTTCCCCTGCTGGTGAAATGTGGAACAGTTGGTTCGATG
GTGAAAGTGTCAGTGATGACTTCATGGCAGAGCGCGAGCAGCCAGTTGAACAGCTGCGCGAATCACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8LU44