Detailed information of TA system TA065784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 454731..455290
• Replicon: chromosome
• Accession: NZ_CP016988
• Organism: Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D7HHK2
• Locus tag: FORC55_RS16825
• Protein ID: WP_000578471.1
• Coordinates: 454731..455009 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: FORC55_RS16830
• Protein ID: WP_000381183.1
• Coordinates: 455006..455290 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC55_RS16745	449794..450114	-	321	WP_096070608.1	IS66 family insertion sequence hypothetical protein	-
FORC55_RS16750	450204..450554	-	351	Protein_430	IS30 family transposase	-
FORC55_RS16760	450947..451129	+	183	WP_000947519.1	DUF645 family protein	-
FORC55_RS16770	451311..451973	+	663	WP_001253122.1	hypothetical protein	-
FORC55_RS16780	452127..452486	+	360	WP_001071525.1	VOC family protein	-
FORC55_RS16790	452790..452961	+	172	Protein_434	DUF645 family protein	-
FORC55_RS16800	453159..453500	+	342	WP_000452085.1	hypothetical protein	-
FORC55_RS16810	453647..454186	+	540	WP_000884557.1	hydrolase	-
FORC55_RS16825	454731..455009	-	279	WP_000578471.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
FORC55_RS16830	455006..455290	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FORC55_RS16835	455494..455991	+	498	WP_000259910.1	GNAT family N-acetyltransferase	-
FORC55_RS16850	456645..456926	+	282	WP_080033233.1	DUF1289 domain-containing protein	-
FORC55_RS16865	457305..458237	+	933	WP_001267735.1	hypothetical protein	-
FORC55_RS16870	458221..458649	+	429	WP_000082746.1	hypothetical protein	-
FORC55_RS16875	458867..459124	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FORC55_RS16880	459112..459414	+	303	WP_000818171.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FORC55_RS16885	459884..460009	+	126	WP_001933211.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	402484..460316	57832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10838.56 Da
• Isoelectric Point: 4.6794

>T65784 WP_000578471.1 NZ_CP016988:c455009-454731 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAEKTDELIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T65784 NZ_CP016988:c455009-454731 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCCGC
TGAAAAAACGGATGAGCTCATAGAAGCCAAAGTTGAAAATTTGCTTGAGCAACCACTAATGGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTACTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGAGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT65784 WP_000381183.1 NZ_CP016988:c455290-455006 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT65784 NZ_CP016988:c455290-455006 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGTCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: A0A271VKS2

Antitoxin

• AlphaFold DB: A0A067BL33