Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 441464..442230 Replicon chromosome
Accession NZ_CP016988
Organism Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag FORC55_RS16675 Protein ID WP_000982260.1
Coordinates 441464..441961 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag FORC55_RS16680 Protein ID WP_000246253.1
Coordinates 441958..442230 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC55_RS16640 436883..437980 + 1098 Protein_412 PAAR domain-containing protein -
FORC55_RS19885 437980..438384 + 405 WP_000915985.1 hypothetical protein -
FORC55_RS16650 438541..439005 + 465 WP_000392737.1 ASCH domain-containing protein -
FORC55_RS16660 439260..440180 - 921 WP_096070524.1 IS5 family transposase -
FORC55_RS16665 440239..441324 + 1086 WP_096070914.1 DUF4238 domain-containing protein -
FORC55_RS16670 441339..441431 + 93 WP_075042595.1 DUF3265 domain-containing protein -
FORC55_RS16675 441464..441961 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
FORC55_RS16680 441958..442230 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
FORC55_RS16690 442469..442966 + 498 WP_000259902.1 GNAT family N-acetyltransferase -
FORC55_RS19890 443023..443130 + 108 WP_096070915.1 acetyltransferase -
FORC55_RS19895 443723..443894 + 172 Protein_422 DUF645 family protein -
FORC55_RS16715 444130..444480 - 351 WP_001086649.1 hypothetical protein -
FORC55_RS16720 444487..445776 - 1290 WP_001250791.1 hypothetical protein -
FORC55_RS16725 446140..446655 + 516 WP_000343786.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 439260..440180 920
- inside Integron - - 402484..460316 57832


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T65783 WP_000982260.1 NZ_CP016988:c441961-441464 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T65783 NZ_CP016988:c441961-441464 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATTGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCAGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT65783 WP_000246253.1 NZ_CP016988:c442230-441958 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT65783 NZ_CP016988:c442230-441958 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References