Detailed information of TA system TA065782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 420204..420732
• Replicon: chromosome
• Accession: NZ_CP016988
• Organism: Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: FORC55_RS16490
• Protein ID: WP_000221352.1
• Coordinates: 420204..420494 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: FORC55_RS16495
• Protein ID: WP_000213183.1
• Coordinates: 420484..420732 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC55_RS16450	415661..415894	+	234	WP_032475975.1	DUF3709 domain-containing protein	-
FORC55_RS19750	416083..416277	+	195	WP_080286596.1	DUF3709 domain-containing protein	-
FORC55_RS16455	416298..416531	+	234	WP_002033284.1	DUF3709 domain-containing protein	-
FORC55_RS16465	417276..417773	+	498	WP_000259910.1	GNAT family N-acetyltransferase	-
FORC55_RS16470	417894..418814	+	921	WP_004393974.1	IS5 family transposase	-
FORC55_RS16475	418956..419252	+	297	WP_001069046.1	hypothetical protein	-
FORC55_RS16480	419443..419775	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FORC55_RS16485	419762..420076	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
FORC55_RS16490	420204..420494	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC55_RS16495	420484..420732	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FORC55_RS16500	420935..421453	+	519	WP_000698780.1	GNAT family N-acetyltransferase	-
FORC55_RS16505	422093..422380	+	288	WP_032475967.1	hypothetical protein	-
FORC55_RS16510	422677..422841	+	165	WP_001902694.1	DUF645 family protein	-
FORC55_RS16520	423059..423547	+	489	WP_000424241.1	hypothetical protein	-
FORC55_RS16525	423684..424166	+	483	WP_032481646.1	GNAT family N-acetyltransferase	-
FORC55_RS16535	424366..424713	+	348	WP_000885701.1	DUF3024 domain-containing protein	-
FORC55_RS16540	424885..425324	+	440	Protein_395	DUF1311 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	417894..418814	920
	-	inside	Integron	-	-	402484..460316	57832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T65782 WP_000221352.1 NZ_CP016988:c420494-420204 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T65782 NZ_CP016988:c420494-420204 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT65782 WP_000213183.1 NZ_CP016988:c420732-420484 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT65782 NZ_CP016988:c420732-420484 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGCGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8