Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 408555..409321 Replicon chromosome
Accession NZ_CP016988
Organism Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag FORC55_RS16380 Protein ID WP_000982260.1
Coordinates 408555..409052 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag FORC55_RS16385 Protein ID WP_000246253.1
Coordinates 409049..409321 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC55_RS16335 403768..404085 - 318 WP_001180238.1 type II toxin-antitoxin system RelE/ParE family toxin -
FORC55_RS16340 404104..404346 - 243 WP_000557291.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FORC55_RS16345 404683..405771 + 1089 WP_096070909.1 PAAR domain-containing protein -
FORC55_RS16350 405771..406172 + 402 WP_032475969.1 hypothetical protein -
FORC55_RS16355 406503..406655 + 153 WP_032469020.1 DUF645 family protein -
FORC55_RS19880 406806..406938 + 133 Protein_365 general secretion pathway protein GspI -
FORC55_RS16365 407013..407195 + 183 WP_001966054.1 DUF645 family protein -
FORC55_RS16375 407409..408413 + 1005 WP_000964935.1 LD-carboxypeptidase -
FORC55_RS16380 408555..409052 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
FORC55_RS16385 409049..409321 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
FORC55_RS16395 409557..409943 + 387 WP_001015079.1 MmcQ/YjbR family DNA-binding protein -
FORC55_RS16405 410181..410420 + 240 WP_000935785.1 DUF645 family protein -
FORC55_RS16410 410620..411117 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
FORC55_RS16415 411114..411386 - 273 WP_000246252.1 DUF1778 domain-containing protein -
FORC55_RS16425 411620..412171 + 552 WP_001008563.1 VRR-NUC domain-containing protein -
FORC55_RS16435 412699..413199 + 501 WP_096070910.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 402484..460316 57832


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T65779 WP_000982260.1 NZ_CP016988:c409052-408555 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T65779 NZ_CP016988:c409052-408555 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATTGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCAGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT65779 WP_000246253.1 NZ_CP016988:c409321-409049 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT65779 NZ_CP016988:c409321-409049 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References