Detailed information of TA system TA065778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-Phd
• Location: 403768..404346
• Replicon: chromosome
• Accession: NZ_CP016988
• Organism: Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FORC55_RS16335
• Protein ID: WP_001180238.1
• Coordinates: 403768..404085 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A366AI09
• Locus tag: FORC55_RS16340
• Protein ID: WP_000557291.1
• Coordinates: 404104..404346 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC55_RS16305	399891..401000	-	1110	Protein_354	IS66 family transposase	-
FORC55_RS16310	401060..401413	-	354	WP_096070609.1	IS66 family insertion sequence element accessory protein TnpB	-
FORC55_RS16315	401410..401730	-	321	WP_096070608.1	IS66 family insertion sequence hypothetical protein	-
FORC55_RS16320	402159..402500	+	342	WP_002021949.1	hypothetical protein	-
FORC55_RS16325	402484..402912	+	429	WP_000074666.1	hypothetical protein	-
FORC55_RS16330	403076..403627	+	552	WP_000672156.1	hypothetical protein	-
FORC55_RS16335	403768..404085	-	318	WP_001180238.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC55_RS16340	404104..404346	-	243	WP_000557291.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FORC55_RS16345	404683..405771	+	1089	WP_096070909.1	PAAR domain-containing protein	-
FORC55_RS16350	405771..406172	+	402	WP_032475969.1	hypothetical protein	-
FORC55_RS16355	406503..406655	+	153	WP_032469020.1	DUF645 family protein	-
FORC55_RS19880	406806..406938	+	133	Protein_365	general secretion pathway protein GspI	-
FORC55_RS16365	407013..407195	+	183	WP_001966054.1	DUF645 family protein	-
FORC55_RS16375	407409..408413	+	1005	WP_000964935.1	LD-carboxypeptidase	-
FORC55_RS16380	408555..409052	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
FORC55_RS16385	409049..409321	-	273	WP_000246253.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	402484..460316	57832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-93)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12169.01 Da
• Isoelectric Point: 9.7495

>T65778 WP_001180238.1 NZ_CP016988:c404085-403768 [Vibrio cholerae]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKPTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Nucleotide

• Length: 318 bp

>T65778 NZ_CP016988:c404085-403768 [Vibrio cholerae]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGCCGACCTTGCTTTCAGGTTTCCAAACTCTAGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTTGGAAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATAGTCGCAGTATTAGGGCAATCACAACTACCCCAAAAACATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8931.22 Da
• Isoelectric Point: 5.6630

>AT65778 WP_000557291.1 NZ_CP016988:c404346-404104 [Vibrio cholerae]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGAAFLNTL

Nucleotide

• Length: 243 bp

>AT65778 NZ_CP016988:c404346-404104 [Vibrio cholerae]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAGCGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGATTTTGAAGAGCTTGAAGCCATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCGCTTTTCTAAACACCCTT
TGA

Structures

Antitoxin

• AlphaFold DB: A0A366AI09