Detailed information of TA system TA065777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 367140..367678
• Replicon: chromosome
• Accession: NZ_CP016988
• Organism: Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FORC55_RS15960
• Protein ID: WP_096070894.1
• Coordinates: 367140..367439 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A7Z7YFC5
• Locus tag: FORC55_RS15965
• Protein ID: WP_001107716.1
• Coordinates: 367436..367678 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC55_RS15900	362598..362939	+	342	WP_000452085.1	hypothetical protein	-
FORC55_RS15905	363123..363353	-	231	WP_000838533.1	PAS factor family protein	-
FORC55_RS15910	363581..364111	+	531	WP_096070893.1	GNAT family N-acetyltransferase	-
FORC55_RS15915	364408..364572	+	165	WP_001902694.1	DUF645 family protein	-
FORC55_RS15925	364784..365218	+	435	WP_000256035.1	GNAT family N-acetyltransferase	-
FORC55_RS15930	365378..365842	+	465	WP_000392737.1	ASCH domain-containing protein	-
FORC55_RS15945	366146..366310	+	165	WP_001902694.1	DUF645 family protein	-
FORC55_RS19720	366451..366636	+	186	WP_157732820.1	DUF3709 domain-containing protein	-
FORC55_RS15955	366657..366890	+	234	WP_002033284.1	DUF3709 domain-containing protein	-
FORC55_RS15960	367140..367439	-	300	WP_096070894.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC55_RS15965	367436..367678	-	243	WP_001107716.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FORC55_RS15975	367973..368398	+	426	WP_029628792.1	hypothetical protein	-
FORC55_RS15980	368550..369143	+	594	WP_096070895.1	hypothetical protein	-
FORC55_RS15985	369606..369731	+	126	WP_001933211.1	DUF3265 domain-containing protein	-
FORC55_RS15990	369764..370327	+	564	WP_096070896.1	hypothetical protein	-
FORC55_RS16000	370470..370985	+	516	WP_096070897.1	lipocalin family protein	-
FORC55_RS19725	371287..371493	+	207	WP_113609612.1	DUF645 family protein	-
FORC55_RS16025	371687..371908	+	222	WP_069213624.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	361389..397418	36029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11684.35 Da
• Isoelectric Point: 9.5980

>T65777 WP_096070894.1 NZ_CP016988:c367439-367140 [Vibrio cholerae]
MRPFQLTNKAKSDLRDIALFTSRRWGHEQRNIYLKQFDDSFWLLAENPDIGKACDEIRDGYRKFPQGSHVIFYRQIGSQN
IEIIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T65777 NZ_CP016988:c367439-367140 [Vibrio cholerae]
ATGAGACCATTTCAGTTAACTAACAAAGCGAAATCTGATTTAAGAGATATAGCCTTGTTTACCTCTCGACGTTGGGGTCA
CGAACAGCGAAATATTTATCTAAAACAGTTTGATGACTCATTTTGGCTGTTAGCCGAAAATCCTGACATTGGTAAAGCCT
GTGACGAAATTCGCGATGGCTACAGAAAGTTTCCACAAGGAAGCCACGTAATATTTTATCGTCAGATCGGCAGTCAAAAT
ATAGAGATTATCCGTATCCTCCACAAAAGCATGGATGTGAATCCAATTTTTGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8895.84 Da
• Isoelectric Point: 4.2950

>AT65777 WP_001107716.1 NZ_CP016988:c367678-367436 [Vibrio cholerae]
MAKNTSITLGDHFDGFIANQIQSGRYGSASEVIRSALRLLETQETKMNTLRQLLVAGEESGVADYDLDSFINELDSEERK

Nucleotide

• Length: 243 bp

>AT65777 NZ_CP016988:c367678-367436 [Vibrio cholerae]
ATGGCTAAGAACACTAGTATTACTTTGGGTGACCATTTTGACGGTTTTATTGCTAATCAAATCCAAAGCGGCCGCTATGG
CTCTGCAAGTGAAGTTATTCGTTCTGCTTTACGTTTACTTGAAACCCAAGAAACTAAAATGAACACTTTACGCCAGTTGC
TTGTTGCGGGCGAAGAAAGTGGAGTTGCGGATTATGACCTTGATTCGTTTATCAATGAGTTAGATAGCGAAGAAAGAAAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z7YFC5