Detailed information of TA system TA065776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2400687..2401456
• Replicon: chromosome
• Accession: NZ_CP016987
• Organism: Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: FORC55_RS11295
• Protein ID: WP_096070663.1
• Coordinates: 2400944..2401456 (+)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: FORC55_RS11290
• Protein ID: WP_000212004.1
• Coordinates: 2400687..2400953 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC55_RS11270	2396391..2396903	-	513	WP_172689549.1	S26 family signal peptidase	-
FORC55_RS11275	2396887..2397234	-	348	WP_077671741.1	plasmid-related protein	-
FORC55_RS11280	2397227..2399626	-	2400	WP_096070661.1	type IV secretion system protein TraC	-
FORC55_RS11285	2399626..2400318	-	693	WP_096070662.1	DsbC family protein	-
FORC55_RS11290	2400687..2400953	+	267	WP_000212004.1	DUF1778 domain-containing protein	Antitoxin
FORC55_RS11295	2400944..2401456	+	513	WP_096070663.1	GNAT family N-acetyltransferase	Toxin
FORC55_RS11300	2401492..2401878	-	387	WP_000180225.1	hypothetical protein	-
FORC55_RS11305	2401875..2402447	-	573	WP_024008834.1	type IV conjugative transfer system lipoprotein TraV	-
FORC55_RS11310	2402522..2403811	-	1290	WP_096070664.1	TraB/VirB10 family protein	-
FORC55_RS11315	2403811..2404710	-	900	WP_096070810.1	type-F conjugative transfer system secretin TraK	-
FORC55_RS11320	2404694..2405320	-	627	WP_096070665.1	pilus assembly protein	-
FORC55_RS11325	2405317..2405598	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2279843..2423108	143265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-83)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 18719.63 Da
• Isoelectric Point: 8.7083

>T65776 WP_096070663.1 NZ_CP016987:2400944-2401456 [Vibrio cholerae]
MGISAPTLLTDEHQLNAFQCGIEELDTWLRKQALKNQKRGTARVYVVNDTEVHQVIGYYAIAMGSVSREQAFSSLRRNSP
DPIPMVILARLGVDKAHQGQGIAAGLLKDCIIRSVQAMNAVGGAGILVHAIDDTAQTFYKKFGFKESTFDPLVLMARICD
IEKSLSTRLR

Nucleotide

• Length: 513 bp

>T65776 NZ_CP016987:2400944-2401456 [Vibrio cholerae]
TTGGGAATAAGTGCACCAACCCTCCTCACTGATGAGCACCAGCTTAATGCCTTTCAATGTGGCATCGAAGAACTCGATAC
TTGGTTAAGAAAGCAGGCGTTAAAGAATCAAAAGCGGGGAACCGCGAGAGTGTATGTGGTCAATGACACGGAAGTACATC
AAGTGATTGGGTACTATGCCATTGCCATGGGATCAGTTTCCAGAGAGCAAGCCTTCAGTTCATTAAGAAGAAATAGTCCA
GATCCCATTCCCATGGTGATTCTAGCCAGGCTTGGAGTAGATAAAGCCCATCAAGGTCAAGGCATTGCAGCCGGTCTTTT
AAAAGATTGTATCATTCGCTCAGTACAAGCAATGAACGCTGTCGGTGGAGCAGGAATTTTAGTTCATGCTATTGATGACA
CTGCGCAAACGTTCTACAAGAAATTTGGCTTCAAAGAATCGACGTTTGATCCTTTAGTACTCATGGCAAGGATCTGCGAT
ATCGAAAAATCATTGTCGACAAGACTGAGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9956.48 Da
• Isoelectric Point: 5.8580

>AT65776 WP_000212004.1 NZ_CP016987:2400687-2400953 [Vibrio cholerae]
MATKTAPINMRVLPSVRDIIDAAASLKKVDRTVFIQQAALNEAHSILAEQRDFVLEAKAFEAFDNELRAEPQTLEGMKDL
FKRKAPWE

Nucleotide

• Length: 267 bp

>AT65776 NZ_CP016987:2400687-2400953 [Vibrio cholerae]
ATGGCTACAAAGACAGCCCCAATCAACATGCGAGTACTGCCATCGGTAAGAGACATCATCGATGCCGCAGCAAGTTTAAA
AAAAGTTGATAGAACCGTGTTCATTCAGCAAGCAGCGCTCAATGAAGCGCATAGTATACTGGCAGAACAGCGGGATTTTG
TTCTAGAAGCAAAGGCTTTTGAAGCGTTTGATAACGAACTTCGTGCAGAGCCACAGACTCTTGAAGGTATGAAAGATCTG
TTTAAGAGGAAAGCGCCTTGGGAATAA