65774

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 961036..961595 Replicon chromosome
Accession NZ_CP016983
Organism Brucella melitensis strain 2008724259

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag BFL33_RS04680 Protein ID WP_002966807.1
Coordinates 961350..961595 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag BFL33_RS04675 Protein ID WP_004683740.1
Coordinates 961036..961350 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL33_RS04630 956741..956944 - 204 WP_004683737.1 hypothetical protein -
BFL33_RS04635 956947..957162 - 216 WP_005968947.1 hypothetical protein -
BFL33_RS04640 957159..957362 - 204 WP_002964088.1 hypothetical protein -
BFL33_RS04645 957412..958116 + 705 WP_005968944.1 hypothetical protein -
BFL33_RS04650 958113..958343 + 231 WP_002964091.1 hypothetical protein -
BFL33_RS04655 958340..958615 + 276 WP_002964092.1 hypothetical protein -
BFL33_RS04660 958638..959114 + 477 WP_002966806.1 hypothetical protein -
BFL33_RS04665 959460..960170 + 711 WP_004685621.1 porin family protein -
BFL33_RS04670 960518..960688 - 171 WP_002964095.1 DUF1508 domain-containing protein -
BFL33_RS04675 961036..961350 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
BFL33_RS04680 961350..961595 - 246 WP_002966807.1 BrnT family toxin Toxin
BFL33_RS04685 961728..962366 + 639 Protein_923 tRNA-guanine transglycosylase -
BFL33_RS17510 962436..963194 + 759 Protein_924 IS5 family transposase -
BFL33_RS04695 963196..963969 + 774 WP_002967634.1 hypothetical protein -
BFL33_RS04700 964300..965751 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 954969..969067 14098
- flank IS/Tn - - 962436..962804 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T65774 WP_002966807.1 NZ_CP016983:c961595-961350 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T65774 NZ_CP016983:c961595-961350 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT65774 WP_004683740.1 NZ_CP016983:c961350-961036 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT65774 NZ_CP016983:c961350-961036 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References