Detailed information of TA system TA065773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943167..943726
• Replicon: chromosome
• Accession: NZ_CP016981
• Organism: Brucella suis strain 2004000577

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BFL32_RS04520
• Protein ID: WP_004688321.1
• Coordinates: 943481..943726 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BFL32_RS04515
• Protein ID: WP_002964097.1
• Coordinates: 943167..943481 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL32_RS04470	938857..939060	-	204	WP_004690812.1	hypothetical protein	-
BFL32_RS04475	939063..939278	-	216	WP_002971459.1	hypothetical protein	-
BFL32_RS04480	939275..939478	-	204	WP_002964088.1	hypothetical protein	-
BFL32_RS04485	939528..940247	+	720	WP_006072696.1	hypothetical protein	-
BFL32_RS04490	940244..940474	+	231	WP_002964091.1	hypothetical protein	-
BFL32_RS04495	940471..940746	+	276	WP_002964092.1	hypothetical protein	-
BFL32_RS04500	940769..941356	+	588	WP_004683738.1	hypothetical protein	-
BFL32_RS04505	941591..942301	+	711	WP_004683739.1	porin family protein	-
BFL32_RS04510	942649..942819	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BFL32_RS04515	943167..943481	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BFL32_RS04520	943481..943726	-	246	WP_004688321.1	BrnT family toxin	Toxin
BFL32_RS04525	943859..944497	+	639	Protein_891	tRNA-guanine transglycosylase	-
BFL32_RS17290	944567..945325	+	759	Protein_892	IS5 family transposase	-
BFL32_RS04535	945326..946099	+	774	WP_006132649.1	hypothetical protein	-
BFL32_RS04540	946234..947880	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	937085..951196	14111
	-	flank	IS/Tn	-	-	944567..944935	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T65773 WP_004688321.1 NZ_CP016981:c943726-943481 [Brucella suis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T65773 NZ_CP016981:c943726-943481 [Brucella suis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT65773 WP_002964097.1 NZ_CP016981:c943481-943167 [Brucella suis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT65773 NZ_CP016981:c943481-943167 [Brucella suis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA