Detailed information of TA system TA065772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 959236..959795
• Replicon: chromosome
• Accession: NZ_CP016979
• Organism: Brucella sp. 2002734562

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: BFS01_RS04760
• Protein ID: WP_002966807.1
• Coordinates: 959550..959795 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BFS01_RS04755
• Protein ID: WP_002964097.1
• Coordinates: 959236..959550 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFS01_RS04700	954928..955131	-	204	WP_002964087.1	hypothetical protein	-
BFS01_RS04705	955134..955349	-	216	WP_002971459.1	hypothetical protein	-
BFS01_RS04710	955346..955549	-	204	WP_002964088.1	hypothetical protein	-
BFS01_RS16485	955599..956317	+	719	Protein_918	hypothetical protein	-
BFS01_RS04725	956314..956544	+	231	WP_002964091.1	hypothetical protein	-
BFS01_RS04730	956541..956816	+	276	WP_002964092.1	hypothetical protein	-
BFS01_RS04735	956839..957315	+	477	WP_002966806.1	hypothetical protein	-
BFS01_RS16490	957661..958370	+	710	Protein_922	porin family protein	-
BFS01_RS04750	958718..958888	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BFS01_RS04755	959236..959550	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BFS01_RS04760	959550..959795	-	246	WP_002966807.1	BrnT family toxin	Toxin
BFS01_RS04765	959928..960566	+	639	Protein_926	tRNA-guanine transglycosylase	-
BFS01_RS17625	960636..961393	+	758	WP_102040076.1	IS5 family transposase	-
BFS01_RS17630	961394..962167	+	774	Protein_928	hypothetical protein	-
BFS01_RS04790	962496..963947	+	1452	WP_004685623.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	953916..967263	13347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T65772 WP_002966807.1 NZ_CP016979:c959795-959550 [Brucella sp. 2002734562]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T65772 NZ_CP016979:c959795-959550 [Brucella sp. 2002734562]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT65772 WP_002964097.1 NZ_CP016979:c959550-959236 [Brucella sp. 2002734562]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT65772 NZ_CP016979:c959550-959236 [Brucella sp. 2002734562]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14