Detailed information of TA system TA065771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 942569..943128
• Replicon: chromosome
• Accession: NZ_CP016977
• Organism: Brucella canis strain 2010009751

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BFL30_RS04530
• Protein ID: WP_004688321.1
• Coordinates: 942883..943128 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BFL30_RS04525
• Protein ID: WP_002964097.1
• Coordinates: 942569..942883 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL30_RS04480	938259..938462	-	204	WP_004690812.1	hypothetical protein	-
BFL30_RS04485	938465..938680	-	216	WP_002971459.1	hypothetical protein	-
BFL30_RS04490	938677..938880	-	204	WP_002964088.1	hypothetical protein	-
BFL30_RS04495	938930..939649	+	720	WP_006072696.1	hypothetical protein	-
BFL30_RS04500	939646..939876	+	231	WP_002964091.1	hypothetical protein	-
BFL30_RS04505	939873..940148	+	276	WP_002964092.1	hypothetical protein	-
BFL30_RS04510	940171..940758	+	588	WP_004683738.1	hypothetical protein	-
BFL30_RS04515	940993..941703	+	711	Protein_888	porin family protein	-
BFL30_RS04520	942051..942221	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BFL30_RS04525	942569..942883	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BFL30_RS04530	942883..943128	-	246	WP_004688321.1	BrnT family toxin	Toxin
BFL30_RS04535	943261..943899	+	639	Protein_892	tRNA-guanine transglycosylase	-
BFL30_RS17080	943969..944726	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
BFL30_RS04550	944727..945500	+	774	WP_006132649.1	hypothetical protein	-
BFL30_RS04555	945635..947281	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	936487..950597	14110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T65771 WP_004688321.1 NZ_CP016977:c943128-942883 [Brucella canis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T65771 NZ_CP016977:c943128-942883 [Brucella canis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT65771 WP_002964097.1 NZ_CP016977:c942883-942569 [Brucella canis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT65771 NZ_CP016977:c942883-942569 [Brucella canis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA