Detailed information of TA system TA065770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 936111..936670
• Replicon: chromosome
• Accession: NZ_CP016973
• Organism: Brucella canis strain 2009004498

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BFL29_RS04490
• Protein ID: WP_004688321.1
• Coordinates: 936425..936670 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BFL29_RS04485
• Protein ID: WP_002964097.1
• Coordinates: 936111..936425 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL29_RS04440	931800..932003	-	204	WP_004690812.1	hypothetical protein	-
BFL29_RS04445	932006..932221	-	216	WP_002971459.1	hypothetical protein	-
BFL29_RS04450	932218..932421	-	204	WP_002964088.1	hypothetical protein	-
BFL29_RS04455	932471..933190	+	720	WP_006072696.1	hypothetical protein	-
BFL29_RS04460	933187..933417	+	231	WP_002964091.1	hypothetical protein	-
BFL29_RS04465	933414..933689	+	276	WP_002964092.1	hypothetical protein	-
BFL29_RS04470	933712..934299	+	588	WP_004683738.1	hypothetical protein	-
BFL29_RS04475	934534..935245	+	712	Protein_877	porin family protein	-
BFL29_RS04480	935593..935763	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BFL29_RS04485	936111..936425	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BFL29_RS04490	936425..936670	-	246	WP_004688321.1	BrnT family toxin	Toxin
BFL29_RS04495	936803..937441	+	639	Protein_881	tRNA-guanine transglycosylase	-
BFL29_RS17215	937511..938268	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
BFL29_RS04510	938269..939042	+	774	WP_006132649.1	hypothetical protein	-
BFL29_RS04515	939177..940823	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	930028..944139	14111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T65770 WP_004688321.1 NZ_CP016973:c936670-936425 [Brucella canis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T65770 NZ_CP016973:c936670-936425 [Brucella canis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT65770 WP_002964097.1 NZ_CP016973:c936425-936111 [Brucella canis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT65770 NZ_CP016973:c936425-936111 [Brucella canis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA