Detailed information of TA system TA065766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3719786..3720460
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: BB988_RS18320
• Protein ID: WP_003417282.1
• Coordinates: 3719786..3720214 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: BB988_RS18325
• Protein ID: WP_003417286.1
• Coordinates: 3720218..3720460 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS18295	3715608..3716009	-	402	WP_003417264.1	cytidine deaminase	-
BB988_RS18300	3716150..3716584	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
BB988_RS18305	3716581..3717015	+	435	WP_009936281.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
BB988_RS18310	3717144..3718916	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
BB988_RS18315	3718916..3719707	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
BB988_RS18320	3719786..3720214	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB988_RS18325	3720218..3720460	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
BB988_RS18330	3720582..3721196	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
BB988_RS18335	3721193..3721858	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
BB988_RS18340	3721859..3722392	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
BB988_RS22405	3722389..3722541	-	153	WP_003916129.1	hypothetical protein	-
BB988_RS18355	3722577..3723838	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T65766 WP_003417282.1 NZ_CP016972:c3720214-3719786 [Mycobacterium tuberculosis H37Ra]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T65766 NZ_CP016972:c3720214-3719786 [Mycobacterium tuberculosis H37Ra]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT65766 WP_003417286.1 NZ_CP016972:c3720460-3720218 [Mycobacterium tuberculosis H37Ra]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT65766 NZ_CP016972:c3720460-3720218 [Mycobacterium tuberculosis H37Ra]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59