Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3149784..3150388 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag BB988_RS15615 Protein ID WP_003414492.1
Coordinates 3149784..3150176 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P71622
Locus tag BB988_RS15620 Protein ID WP_003912003.1
Coordinates 3150173..3150388 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS15585 3144934..3145722 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
BB988_RS15590 3146056..3146601 - 546 WP_003913758.1 DUF1802 family protein -
BB988_RS15595 3146873..3147757 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
BB988_RS15600 3147760..3148647 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
BB988_RS15605 3148952..3149497 - 546 WP_003899500.1 DUF1802 family protein -
BB988_RS15610 3149494..3149763 - 270 WP_003414489.1 DUF2277 family protein -
BB988_RS15615 3149784..3150176 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
BB988_RS15620 3150173..3150388 - 216 WP_003912003.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BB988_RS15625 3150435..3151184 + 750 WP_003414497.1 enoyl-CoA hydratase -
BB988_RS15630 3151263..3152345 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
BB988_RS15635 3152338..3153648 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
BB988_RS15640 3153651..3154478 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
BB988_RS15645 3154475..3155386 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T65762 WP_003414492.1 NZ_CP016972:c3150176-3149784 [Mycobacterium tuberculosis H37Ra]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T65762 NZ_CP016972:c3150176-3149784 [Mycobacterium tuberculosis H37Ra]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7483.50 Da        Isoelectric Point: 4.9732

>AT65762 WP_003912003.1 NZ_CP016972:c3150388-3150173 [Mycobacterium tuberculosis H37Ra]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Download         Length: 216 bp

>AT65762 NZ_CP016972:c3150388-3150173 [Mycobacterium tuberculosis H37Ra]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB P71622

References