Detailed information of TA system TA065758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3083334..3084013
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: BB988_RS15235
• Protein ID: WP_003414059.1
• Coordinates: 3083334..3083750 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BB988_RS15240
• Protein ID: WP_003414061.1
• Coordinates: 3083747..3084013 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS15215	3079386..3080288	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BB988_RS15220	3080357..3081109	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BB988_RS15225	3081353..3081628	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BB988_RS15230	3081625..3083247	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
BB988_RS15235	3083334..3083750	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
BB988_RS15240	3083747..3084013	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BB988_RS15245	3084039..3084434	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
BB988_RS15250	3084431..3084700	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BB988_RS15255	3084710..3085804	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
BB988_RS15260	3085801..3086220	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
BB988_RS15265	3086294..3086773	-	480	WP_003414073.1	dihydrofolate reductase	-
BB988_RS15270	3086844..3087644	-	801	WP_003911953.1	thymidylate synthase	-
BB988_RS15275	3087800..3088537	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T65758 WP_003414059.1 NZ_CP016972:c3083750-3083334 [Mycobacterium tuberculosis H37Ra]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T65758 NZ_CP016972:c3083750-3083334 [Mycobacterium tuberculosis H37Ra]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT65758 WP_003414061.1 NZ_CP016972:c3084013-3083747 [Mycobacterium tuberculosis H37Ra]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT65758 NZ_CP016972:c3084013-3083747 [Mycobacterium tuberculosis H37Ra]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64