Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2943234..2943948 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag BB988_RS14395 Protein ID WP_003413460.1
Coordinates 2943508..2943948 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag BB988_RS14390 Protein ID WP_003413456.1
Coordinates 2943234..2943521 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS14355 2938656..2938901 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BB988_RS14360 2938898..2939302 + 405 WP_003899389.1 type II toxin-antitoxin system VapC family toxin -
BB988_RS14365 2939519..2940139 + 621 WP_003413441.1 DUF4178 domain-containing protein -
BB988_RS14370 2940150..2940644 + 495 WP_003413444.1 DUF2617 family protein -
BB988_RS14375 2940641..2941072 + 432 WP_003899390.1 DUF4247 domain-containing protein -
BB988_RS14380 2941127..2941555 + 429 WP_178121172.1 DUF350 domain-containing protein -
BB988_RS14385 2941552..2943123 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
BB988_RS14390 2943234..2943521 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
BB988_RS14395 2943508..2943948 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
BB988_RS14400 2943969..2944724 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
BB988_RS14405 2944857..2945453 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
BB988_RS14410 2945461..2946306 - 846 WP_003413466.1 acyl-CoA thioesterase II -
BB988_RS14415 2946335..2947234 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
BB988_RS14420 2947362..2948036 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T65756 WP_003413460.1 NZ_CP016972:2943508-2943948 [Mycobacterium tuberculosis H37Ra]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T65756 NZ_CP016972:2943508-2943948 [Mycobacterium tuberculosis H37Ra]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT65756 WP_003413456.1 NZ_CP016972:2943234-2943521 [Mycobacterium tuberculosis H37Ra]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT65756 NZ_CP016972:2943234-2943521 [Mycobacterium tuberculosis H37Ra]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References