Detailed information of TA system TA065754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2881770..2882401
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: BB988_RS14095
• Protein ID: WP_003413174.1
• Coordinates: 2882024..2882401 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: BB988_RS14090
• Protein ID: WP_003413167.1
• Coordinates: 2881770..2882027 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS14055	2877684..2877998	+	315	WP_009937839.1	hypothetical protein	-
BB988_RS14060	2878294..2879505	+	1212	WP_009936235.1	alpha/beta hydrolase family protein	-
BB988_RS14070	2879632..2880291	+	660	WP_003900846.1	LppA family lipoprotein	-
BB988_RS14075	2880288..2880950	+	663	WP_003900848.1	hypothetical protein	-
BB988_RS14080	2880947..2881225	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
BB988_RS14085	2881318..2881731	+	414	WP_003413164.1	PIN domain nuclease	-
BB988_RS14090	2881770..2882027	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
BB988_RS14095	2882024..2882401	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB988_RS14100	2882417..2882791	-	375	WP_003413177.1	hypothetical protein	-
BB988_RS14105	2882891..2883286	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
BB988_RS14110	2883283..2883528	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
BB988_RS14120	2883939..2884358	-	420	WP_003413190.1	A24 family peptidase	-
BB988_RS14125	2884370..2885179	-	810	WP_003413193.1	shikimate dehydrogenase	-
BB988_RS14130	2885176..2886429	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
BB988_RS14135	2886422..2886934	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T65754 WP_003413174.1 NZ_CP016972:2882024-2882401 [Mycobacterium tuberculosis H37Ra]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T65754 NZ_CP016972:2882024-2882401 [Mycobacterium tuberculosis H37Ra]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT65754 WP_003413167.1 NZ_CP016972:2881770-2882027 [Mycobacterium tuberculosis H37Ra]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT65754 NZ_CP016972:2881770-2882027 [Mycobacterium tuberculosis H37Ra]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5