Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2881770..2882401 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BB988_RS14095 Protein ID WP_003413174.1
Coordinates 2882024..2882401 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BB988_RS14090 Protein ID WP_003413167.1
Coordinates 2881770..2882027 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS14055 2877684..2877998 + 315 WP_009937839.1 hypothetical protein -
BB988_RS14060 2878294..2879505 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
BB988_RS14070 2879632..2880291 + 660 WP_003900846.1 LppA family lipoprotein -
BB988_RS14075 2880288..2880950 + 663 WP_003900848.1 hypothetical protein -
BB988_RS14080 2880947..2881225 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BB988_RS14085 2881318..2881731 + 414 WP_003413164.1 PIN domain nuclease -
BB988_RS14090 2881770..2882027 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
BB988_RS14095 2882024..2882401 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BB988_RS14100 2882417..2882791 - 375 WP_003413177.1 hypothetical protein -
BB988_RS14105 2882891..2883286 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BB988_RS14110 2883283..2883528 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BB988_RS14120 2883939..2884358 - 420 WP_003413190.1 A24 family peptidase -
BB988_RS14125 2884370..2885179 - 810 WP_003413193.1 shikimate dehydrogenase -
BB988_RS14130 2885176..2886429 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BB988_RS14135 2886422..2886934 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T65754 WP_003413174.1 NZ_CP016972:2882024-2882401 [Mycobacterium tuberculosis H37Ra]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T65754 NZ_CP016972:2882024-2882401 [Mycobacterium tuberculosis H37Ra]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT65754 WP_003413167.1 NZ_CP016972:2881770-2882027 [Mycobacterium tuberculosis H37Ra]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT65754 NZ_CP016972:2881770-2882027 [Mycobacterium tuberculosis H37Ra]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References